Raw Data, Win MN, Smolke CD. 2008. Higher-order cellular information processing with synthetic RNA devices. Science. 322: 456-60. DOI: 10.1126/science.1160311

Abstract

This supplement provides additional detail on the data analysis methods and the raw data for the RNA devices presented in the published manuscript and supporting online material. The information provided in the current supplement is organized as follows:

1. Gating methods for the raw flow cytometry data 2. Correction methods for nonspecific effects on fluorescence of chemical effectors controlling the RNA devices 3. Selection of standard against which to report device performance 4. Relevance to conclusions published in the Science paper 5. Example calculations 6. Raw data for RNA devices