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BBF RFC 38: RFC 38: Building Blocks - Standard Large DNA/Genome Construction

Research and Teaching Output of the MIT Community

Show simple item record DiCarlo, James Boeke, Jef 2009-10-07T21:51:51Z 2009-10-07T21:51:51Z 2009-10-07T21:51:51Z
dc.description.abstract The physical assembly of standard parts is currently a non-standard process, which can either come from direct genome PCR with restriction enzyme sites incorporated into the PCR primers, or overlap assembly PCR. Furthermore, current Biobrick construction standards [RFC 10,11,12 etc.] rely heavily on restriction enzyme based methods, which can be sequence, cost and time restrictive. This is especially true of large DNA assembly, where 100% control of over DNA sequence may be mandatory, such as in whole genome assembly. We propose a novel standard, The Building Block Method, for both the construction of standard parts and their assembly This Building Block standard can be either A) an interchangeable or B) a non-interchangeable format, based on the desired use of the part. en
dc.language.iso en_US en
dc.relation.ispartofseries BBF RFC;38
dc.subject technical standards en
dc.subject request for comment en
dc.subject biobricks en
dc.subject synthetic biology en
dc.title BBF RFC 38: RFC 38: Building Blocks - Standard Large DNA/Genome Construction en
dc.type Technical Report en

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