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BBF RFC 47: BioBytes Assembly Standard

Research and Teaching Output of the MIT Community

Show simple item record Ellison, Michael Ridgway, Doug Fedor, Justin Garside, Erin Robinson, Kelly Lloyd, David 2009-10-29T23:42:34Z 2009-10-29T23:42:34Z 2009-10-29T23:42:34Z
dc.description.abstract The BioBytes assembly method provides a new high-throughput way of assembling plasmids as well as other large DNA constructs. Using a bead-linked assembly of genetic parts with long (12 bp) standardized sticky ends, it allows for greater speed and efficiency in assembling these components. This document defines a standard for genetic parts compatible with the assembly system and describes detailed protocols for the construction of such parts and their rapid assembly. Although the original motivation for the method was to produce whole synthetic genomes, the design allows for any type of DNA construction. en
dc.language.iso en_US en
dc.relation.ispartofseries BBF RFC;47
dc.subject synthetic biology en
dc.subject standard biological parts en
dc.title BBF RFC 47: BioBytes Assembly Standard en
dc.type Technical Report en

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