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BBF RFC 47: BioBytes Assembly Standard

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dc.contributor.author Ellison, Michael
dc.contributor.author Ridgway, Doug
dc.contributor.author Fedor, Justin
dc.contributor.author Garside, Erin
dc.contributor.author Robinson, Kelly
dc.contributor.author Lloyd, David
dc.date.accessioned 2009-10-29T23:42:34Z
dc.date.available 2009-10-29T23:42:34Z
dc.date.issued 2009-10-29T23:42:34Z
dc.identifier.uri http://hdl.handle.net/1721.1/49518
dc.description.abstract The BioBytes assembly method provides a new high-throughput way of assembling plasmids as well as other large DNA constructs. Using a bead-linked assembly of genetic parts with long (12 bp) standardized sticky ends, it allows for greater speed and efficiency in assembling these components. This document defines a standard for genetic parts compatible with the assembly system and describes detailed protocols for the construction of such parts and their rapid assembly. Although the original motivation for the method was to produce whole synthetic genomes, the design allows for any type of DNA construction. en
dc.language.iso en_US en
dc.relation.ispartofseries BBF RFC;47
dc.subject synthetic biology en
dc.subject standard biological parts en
dc.title BBF RFC 47: BioBytes Assembly Standard en
dc.type Technical Report en


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