Fast multiple gene fragment ligation method based on Type IIs restriction enzyme DraIII
Citable URI:
http://hdl.handle.net/1721.1/59802
| Title: | Fast multiple gene fragment ligation method based on Type IIs restriction enzyme DraIII |
| Author: | Shi, Zhenyu; Li, Teng; Chen, Guoqiang |
| Issue Date: | 2010-10-31 |
| Abstract: | With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 61 is developed aimed at completing the ligation of multiple fragments quickly and efficiently based on Type II restriction enzyme DraIII. |
| URI: | http://hdl.handle.net/1721.1/59802 |
| Series/Report no.: | BBF RFC;61 |
| Keywords: | assembly, method |
Files in this item
| Files | Size | Format | View | |
|---|---|---|---|---|
| BBFRFC61.pdf | 292.3Kb |
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