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Fast multiple gene fragment ligation method based on homologous recombination

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Fast multiple gene fragment ligation method based on homologous recombination

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Author: Wang, Ruiyan; Shi, Zhenyu; Li, Teng; Chen, Guoqiang
Date Issued: 2010-10-31
Abstract:
With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 62 is developed aimed at completing the ligation of multiple fragments quickly and efficiently based on homologous recombination.
URI: http://hdl.handle.net/1721.1/59803
Series/Report no.: BBF RFC;62
Keywords: assembly, method

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