Measurement of bacterial promoter absolute activity and determination of dynamic performance in cell-free expression system by qPCR
Citable URI:
http://hdl.handle.net/1721.1/60093
| Title: | Measurement of bacterial promoter absolute activity and determination of dynamic performance in cell-free expression system by qPCR |
| Author: | Olchowik, Anna; Lower, Michal; Moreno, Cherry; Pankowski, Jaroslaw |
| Issue Date: | 2010-12-05 |
| Abstract: | The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of bacterial promoters. The absolute activity can be experimentally measured in Moles of RNA per Moles of DNA in time, and therefore it is can be expressed in PoPS [1] |
| URI: | http://hdl.handle.net/1721.1/60093 |
| Series/Report no.: | BBF RFC;58 |
| Keywords: | measurement |
Files in this item
| Files | Size | Format | View | |
|---|---|---|---|---|
| BBFRFC58.pdf | 90.21Kb |
View/ |
The following license files are associated with this item:
This item appears in the following Collection(s)
Except where otherwise noted, this item's license is described as Attribution-No Derivative Works 3.0 United States
All Items in DSpace@MIT are protected by original copyright, with all rights reserved, unless otherwise indicated.
|
|
Contact
MIT Libraries
|