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dc.contributor.authorChe, Austinen_US
dc.date.accessioned2004-10-20T20:32:21Z
dc.date.available2004-10-20T20:32:21Z
dc.date.issued2003-08-31en_US
dc.identifier.otherAITR-2003-017en_US
dc.identifier.urihttp://hdl.handle.net/1721.1/7112
dc.description.abstractTo engineer complex synthetic biological systems will require modular design, assembly, and characterization strategies. The RNA polymerase arrival rate (PAR) is defined to be the rate that RNA polymerases arrive at a specified location on the DNA. Designing and characterizing biological modules in terms of RNA polymerase arrival rates provides for many advantages in the construction and modeling of biological systems. PARMESAN is an in vitro method for measuring polymerase arrival rates using pyrrolo-dC, a fluorescent DNA base that can substitute for cytosine. Pyrrolo-dC shows a detectable fluorescence difference when in single-stranded versus double-stranded DNA. During transcription, RNA polymerase separates the two strands of DNA, leading to a change in the fluorescence of pyrrolo-dC. By incorporating pyrrolo-dC at specific locations in the DNA, fluorescence changes can be taken as a direct measurement of the polymerase arrival rate.en_US
dc.format.extent112 p.en_US
dc.format.extent92476964 bytes
dc.format.extent3362118 bytes
dc.format.mimetypeapplication/postscript
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.relation.ispartofseriesAITR-2003-017en_US
dc.subjectAIen_US
dc.titleFluorescence Assay for Polymerase Arrival Ratesen_US


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