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The GoldenBricks assembly: A standardized one-shot cloning technique for complete cassette assembly

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The GoldenBricks assembly: A standardized one-shot cloning technique for complete cassette assembly

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Author: Pauthenier, Cyrille; El-Sayyed, Hafez; Rostain, William; Cerisy, Tristan; Lopez, Carolina Gallo; Ferreira, Raphael; Moreira, Jorgelindo Da Veiga; Souterre, Tiffany; Eeckhout, Joachim; Nogue, Pierre Yves; Llamosi, Artemis; Parutto, Pierre; Nikolayeva, Iryna; Machat, Mohamed; Marquet, Clement; Młynarczyk, Anna; Thelie, Aurore; Landrain, Thomas; Tolonen, Andrew; Pollet, Nicolas; Faulon, Jean-Loup; Jaramilo, Alfonso
Date Issued: 2012-11-05
Abstract:
BBF RFC 92 proposes a new standard assembly method for the Parts Registry. The method makes one-shot cloning of a complete eukaryotic or prokaryotic cassette possible in one day while keeping compatibility with the BBF RFC 10 BioBrick assembly standard.
URI: http://hdl.handle.net/1721.1/74561
Series/Report no.: BBF RFC;92
Keywords: DNA assembly

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