| dc.contributor.author | Beer, Ralf | |
| dc.contributor.author | Christiansen, Tania | |
| dc.contributor.author | Herbst, Konrad | |
| dc.contributor.author | Ignatiadis, Nikolaos | |
| dc.contributor.author | Kats, Ilia | |
| dc.contributor.author | Kurzawa, Nils | |
| dc.contributor.author | Meichsner, Johanna | |
| dc.contributor.author | Rabe, Sophie | |
| dc.contributor.author | Riedel, Anja | |
| dc.contributor.author | Sachs, Joshua | |
| dc.contributor.author | Schessner, Julia | |
| dc.contributor.author | Schmidt, Florian | |
| dc.contributor.author | Walch, Philipp | |
| dc.contributor.author | Adlung, Lorenz | |
| dc.contributor.author | Genereith, Katharina | |
| dc.contributor.author | Georgi, Fanny | |
| dc.contributor.author | Heinemann, Tim | |
| dc.contributor.author | Meyer, Hannah | |
| dc.contributor.author | Niopek, Dominik | |
| dc.contributor.author | Di Ventura, Barbara | |
| dc.contributor.author | Eils, Roland | |
| dc.date.accessioned | 2013-10-05T00:50:45Z | |
| dc.date.available | 2013-10-05T00:50:45Z | |
| dc.date.issued | 2013-10-04 | |
| dc.identifier.uri | http://hdl.handle.net/1721.1/81332 | |
| dc.description.abstract | The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High Throughput Circular Extension Cloning and Transformation (HiCT).
Description of construct libraries generated by the HiCT method can be found at http://2013.igem.org/Team:Heidelberg/Indigoidine.
This RFC also points out further optimization strategies with regard to construct stability, reduction of transformation background and the generation of competent cells. | en_US |
| dc.language.iso | en | en_US |
| dc.relation.ispartofseries | BBF RFC;99 | |
| dc.rights | Attribution-NoDerivs 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nd/3.0/us/ | * |
| dc.subject | DNA assembly | en_US |
| dc.title | HiCT: High Throughput Protocols For CPE Cloning And Transformation | en_US |
| dc.type | Technical Report | en_US |
