This is an archived course. A more recent version may be available at ocw.mit.edu.

Lecture Notes

The lecture notes linked below are from the Fall 2003 version of this course. The lecture notes were created by Prof. King unless otherwise noted.

SES # TOPICS LECTURE NOTES
1 Introduction to the problem (PDF)
2

Side-chain review

Sulfur redox chemistry

  
3 The Anfinsen experiments (PDF)
4

Globular protein structure

Protein interiors

 (PDF)
5 Using the Protein database (PDB) (PDF - 2.5 MB) (Courtesy of Prof. David Gossard)
6 Helix-helix packing in globular proteins (PDF)
7

Beta-sheets

Beta-sheet packing

 (PDF)
8 Experimental techniques: circular dichroism  
9

Thermal denaturation

Coiled coils

Refolding of tropomyosin

(PDF 1) (Courtesy of Prof. David Gossard)

(PDF 2)
10 Fluorescence spectroscopy  
11 S-peptide helical folding (PDF)
12 Detecting partially folded intermediates (PDF)
13 Prolyl isomerization  
14 Cytochrome c refolding pathway (PDF)
15 2-D NMR techniques  
16 Collagen structure and folding in vivo (PDF - 1.8 MB) (Courtesy of Prof. David Gossard)
17 Procollagen folding in vitro  
18 Protein calorimetry: BPTI  
19 Protein misfolding and aggregation (PDF)
20

Ribosome channel

Nascent chains

Trigger factor

 (PDF)
21 Scaffolding proteins in viral shell assembly  
22 Amyloid fiber formation in neuro degenerative disease  
24 Chaperonin assisted folding (PDF)
25 Eukariotic chaperonins  
26 Paper topic discussion and choices  
27 Membrane protein (rhodopsin) folding and assembly  
28 Prion diseases  
29 Etiology of some human protein deposition diseases