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Lecture Notes

The table below includes an example set of notes taken by a student in the course for each lecture session (L#).

Module 1: Size and Components of Cells and Implications with respect to Regulation
L1 Introduction: cell constituents, prokaryotes vs. eukaryotes (PDF)
L2 Introduction (cont'd) (PDF)
Module 2: Fatty Acid Synthases (FAS), Polyketide Synthases (PKS), and Non-ribosomal Polypeptide Synthases (NRPS)
L3 Fatty Acid Synthase: polymerization, biosynthesis, players, chemistry, structure, chemistry as a paradigm for PKS and NRPS, medical interlude (PDF)
L4 Experimental methods for elucidating FAS structure (PDF)
L5 Chemistry of FAS as paradigm for other molecular machines (PDF)
L6 Secondary metabolism: PKS, NRPS (PDF)
L7 Chemistry of PKS and NRPS: post-translational modification, initiation, elongation, decoration, termination, fidelity (PDF)
L8 Chemistry of PKS and NRPS (cont'd) (PDF)
L9 Chemistry of PKS and NRPS (cont'd with specific examples) (PDF)
L10 Biosynthesis of yersiniabactin and cholesterol (PDF)
L11 Cholesterol biosynthesis (PDF)
L12 Cholesterol regulation and homeostasis (PDF)
L13 Sensing insoluble molecules (PDF)
L14 Module 2: Regulation of the transcription level by insoluble metabolites and Module 3: Translation (PDF)
Module 3: Translation: Loading, Initiation, Elongation, and Termination - A Machine in Action; Introduction to G-proteins: Switches or Motors
L15 Translation (cont'd) (PDF)
L16 Elongation, termination, RNA polymerase (PDF)
L17 Chemical methods for studying translation and the ribosome (PDF)
L18 Chemical methods for studying translation and the ribosome (cont'd) (PDF)
L19 Chemical methods for studying translation and the ribosome (cont'd) (PDF)
L20 Isoleucine tRNA synthetase (PDF)
L21 tRNA synthase editing mechanisms; G proteins (EF-Tu/EF-G) (PDF)
L22 G proteins: motors (PDF)
L23 G proteins: switches (PDF)
L24 Peptide bond formation; new technologies using the ribosome (PDF)
L25 Module 3: methods for the incorporation of unnatural amino acids and Module 4: what happens as a protein exits the ribosome? (PDF)
Module 4: Crypts and Chambers: Macromolecular Machines involved in Protein Folding and Degradation
L26 Protein folding in vitro (PDF)
L27 Protein folding: in vitro vs. in vivo; degradation (PDF)
L28 Protein folding in vivo (PDF)
L29 Chaperone proteins (PDF)
L30 GroEL/GroES (PDF)
L31 Proteases (PDF)
L32 Proteosome (PDF)
L33 Proteosome (cont'd) (PDF)
L34 Role of Ubiquitin in degradation (PDF)
L35 Degradation through polyubiquitination (PDF)