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Biological detection by means of mass reduction in a suspended microchannel resonator
(Massachusetts Institute of Technology, 2004)
Label-free detection is the detection of biomolecules and their interactions, without the use of a molecule external to the interaction, used as a reporter to indicate presence and/or location. The suspended microchannel ...
High-throughput genomic phenotyping
(Massachusetts Institute of Technology, 2004)
In the wake of the development of technology to sequence the complete genome of an organism, it has become expedient to generate methodologies to elucidate and characterize the function of all genes constituting the complete ...
Investigation of growth factors and cytokines that suppress adult stem cell asymmetric cell kinetics
(Massachusetts Institute of Technology, 2005)
Adult stem cells are potentially useful in many biomedical applications that can save lives and increase the quality of a patient's life, such as tissue engineering, cell replacement, and gene therapy. However, these ...
Using optical tweezers, single molecule fluorescence and the ZIF268 protein-DNA system to probe mechanotransduction mechanisms
(Massachusetts Institute of Technology, 2006)
Optical tweezers instruments use laser radiation pressure to trap microscopic dielectric beads. With the appropriate chemistry, such a bead can be attached to a single molecule as a handle, permitting the application of ...
Molecular mechanism of interactions between estrogen receptor and estrogen receptor selective genotoxins
(Massachusetts Institute of Technology, 2000)
Although one million new breast cancer cases arise each year worldwide, therapies to treat the disease are limited. Conventional treatments including the chemotherapeutic agent, Tamoxifen, have had only limited success, ...
Distribution of mutant cells in human skin : exploration of the fetal-juvenile mutability hypothesis
(Massachusetts Institute of Technology, 2009)
The multiple "hits" carcinogenesis models are extensions of the cancer incidence theory developed by researchers from Nordling (1953), Armitage-Doll (1954 and 1957), Knudson (1971), Moolgavkar and Verzon (1979), to Moolgavkar ...
Transcriptional divergence and conservation of human and mouse erythropoiesis
(Massachusetts Institute of Technology, 2014)
Mouse models have been used extensively for decades and have been instrumental in improving our understanding of mammalian erythropoiesis. Nonetheless, there are several examples of variation between human and mouse ...
Developing a robust harvest for high cell density CHO cell culture
(Massachusetts Institute of Technology, 2020)
Genetic requirements for protection against bleomycin toxicity in Escherichia coli
(Massachusetts Institute of Technology, 1999)
Bleomycin is known to cause double strand breaks in vitro. Little is known, however, about its mechanism of genotoxicity in vivo. One way to probe the mechanism of genotoxicity of a DNA damaging agent in vivo is to compare ...
A quantitative proteomics study of the additive effect of inflammatory cytokines and injurious compression on cartilage damage
(Massachusetts Institute of Technology, 2011)
Objectives: 1) To perform a quantitative comparison of proteins released to media on combination with cytokine (IL-1[beta[ or TNF-[alpha]) and Injury as compared to either treatment alone, and to thus identify proteins ...