Synthetic Biology: Recent submissions
Now showing items 52-54 of 139
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Fast multiple gene fragment ligation method based on Type IIs restriction enzyme DraIII
(2010-10-31)With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 61 is developed aimed at ... -
Quantitative measurement of mammalian cell invasion by bacteria using flow cytometry
(2010-10-31)Purpose of this RFC is to provide method of measurement of bacterial invasion into mammalian cells. Also this RFC defines Percent of INvasion (PIN) unit. -
Abbreviated BioBrick Prefix and Suffix for More Efficient Primer Design
(2010-10-31)This Request for Comments (RFC) modifies the assembly standard for biological parts proposed in BBF RFC 10 by removing the NotI restriction site from the BioBrick Prefix and Suffix.


