Assembly of BioBrick standard biological parts using three antibiotic assembly
Author(s)
Shetty, Reshma; Lizarazo, Meagan; Rettberg, Randy; Knight, Thomas F., Jr.
DownloadRevised author's version, compiled (600.7Kb)
Terms of use
Metadata
Show full item recordAbstract
An underlying goal of synthetic biology is to make the process of engineering biological systems easier and more reliable. In support of this goal, we developed BioBrick assembly standard 10 to enable the construction of systems from standardized genetic parts. The BioBrick standard underpins the distributed efforts by the synthetic biology research community to develop a collection of more than 6000 standard genetic parts available from the Registry of Standard Biological Parts. Here, we describe the three antibiotic assembly method for physical composition of BioBrick parts and provide step-by-step protocols. The method relies on a combination of positive and negative selection to eliminate time- and labor-intensive steps such as column cleanup and agarose gel purification of DNA during part assembly.
Description
This is a revised personal version of the text of the final journal article available via DOI: 10.1016/B978-0-12-385120-8.00013-9
Date issued
2011-05-20Publisher
Elsevier Inc.
Citation
Shetty R, Lizarazo M, Rettberg R, Knight TF. Assembly of BioBrick standard biological parts using three antibiotic assembly. Methods Enzymol. 2011;498:311-26.
Other identifiers
PMID:21601683
DOI:10.1016/B978-0-12-385120-8.00013-9
ISBN
978-0-12-385120-8
ISSN
0076-6879
Keywords
biobrick parts, DNA assembly
Collections
The following license files are associated with this item: