dc.contributor.author | Belle, Janeil | |
dc.contributor.author | Ysasi, Alexandra | |
dc.contributor.author | Bennett, Robert D. | |
dc.contributor.author | Filipovic, Nenad | |
dc.contributor.author | Imani Nejad, Mohammad | |
dc.contributor.author | Trumper, David L. | |
dc.contributor.author | Ackermann, Maximilian | |
dc.contributor.author | Wagner, Willi | |
dc.contributor.author | Tsuda, Akira | |
dc.contributor.author | Konerding, Moritz A. | |
dc.contributor.author | Mentzer, Steven J. | |
dc.date.accessioned | 2016-08-11T16:22:26Z | |
dc.date.available | 2016-08-11T16:22:26Z | |
dc.date.issued | 2014-06 | |
dc.identifier.issn | 00262862 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/103899 | |
dc.description.abstract | Vascular systems grow and remodel in response to not only metabolic needs, but also mechanical influences as well. Here, we investigated the influence of tissue-level mechanical forces on the patterning and structure of the chick chorioallantoic membrane (CAM) microcirculation. A dipole stretch field was applied to the CAM using custom computer-controlled servomotors. The topography of the stretch field was mapped using finite element models. After 3 days of stretch, Sholl analysis of the CAM demonstrated a 7-fold increase in conducting vessel intersections within the stretch field (p < 0.01). The morphometric analysis of intravital microscopy and scanning electron microscopy (SEM) images demonstrated that the increase vessel density was a result of an increase in interbranch distance (p < 0.01) and a decrease in bifurcation angles (p < 0.01); there was no significant increase in conducting vessel number (p > 0.05). In contrast, corrosion casting and SEM of the stretch field capillary meshwork demonstrated intense sprouting and intussusceptive angiogenesis. Both planar surface area (p < 0.05) and pillar density (p < 0.01) were significantly increased relative to control regions of the CAM. We conclude that a uniaxial stretch field stimulates the axial growth and realignment of conducting vessels as well as intussusceptive and sprouting angiogenesis within the gas exchange capillaries of the ex ovo CAM. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (NIH grant HL95678) | en_US |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1016/j.mvr.2014.06.009 | en_US |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs License | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
dc.source | PMC | en_US |
dc.title | Stretch-induced intussuceptive and sprouting angiogenesis in the chick chorioallantoic membrane | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Belle, Janeil, Alexandra Ysasi, Robert D. Bennett, Nenad Filipovic, Mohammad Imani Nejad, David L. Trumper, Maximilian Ackermann, et al. “Stretch-Induced Intussuceptive and Sprouting Angiogenesis in the Chick Chorioallantoic Membrane.” Microvascular Research 95 (September 2014): 60–67. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Mechanical Engineering | en_US |
dc.contributor.mitauthor | Imani Nejad, Mohammad | en_US |
dc.contributor.mitauthor | Trumper, David L. | en_US |
dc.relation.journal | Microvascular Research | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Belle, Janeil; Ysasi, Alexandra; Bennett, Robert D.; Filipovic, Nenad; Nejad, Mohammad Imani; Trumper, David L.; Ackermann, Maximilian; Wagner, Willi; Tsuda, Akira; Konerding, Moritz A.; Mentzer, Steven J. | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-8981-1912 | |
dc.identifier.orcid | https://orcid.org/0000-0001-5358-5450 | |
mit.license | PUBLISHER_CC | en_US |