Show simple item record

dc.contributor.authorBelle, Janeil
dc.contributor.authorYsasi, Alexandra
dc.contributor.authorBennett, Robert D.
dc.contributor.authorFilipovic, Nenad
dc.contributor.authorImani Nejad, Mohammad
dc.contributor.authorTrumper, David L.
dc.contributor.authorAckermann, Maximilian
dc.contributor.authorWagner, Willi
dc.contributor.authorTsuda, Akira
dc.contributor.authorKonerding, Moritz A.
dc.contributor.authorMentzer, Steven J.
dc.date.accessioned2016-08-11T16:22:26Z
dc.date.available2016-08-11T16:22:26Z
dc.date.issued2014-06
dc.identifier.issn00262862
dc.identifier.urihttp://hdl.handle.net/1721.1/103899
dc.description.abstractVascular systems grow and remodel in response to not only metabolic needs, but also mechanical influences as well. Here, we investigated the influence of tissue-level mechanical forces on the patterning and structure of the chick chorioallantoic membrane (CAM) microcirculation. A dipole stretch field was applied to the CAM using custom computer-controlled servomotors. The topography of the stretch field was mapped using finite element models. After 3 days of stretch, Sholl analysis of the CAM demonstrated a 7-fold increase in conducting vessel intersections within the stretch field (p < 0.01). The morphometric analysis of intravital microscopy and scanning electron microscopy (SEM) images demonstrated that the increase vessel density was a result of an increase in interbranch distance (p < 0.01) and a decrease in bifurcation angles (p < 0.01); there was no significant increase in conducting vessel number (p > 0.05). In contrast, corrosion casting and SEM of the stretch field capillary meshwork demonstrated intense sprouting and intussusceptive angiogenesis. Both planar surface area (p < 0.05) and pillar density (p < 0.01) were significantly increased relative to control regions of the CAM. We conclude that a uniaxial stretch field stimulates the axial growth and realignment of conducting vessels as well as intussusceptive and sprouting angiogenesis within the gas exchange capillaries of the ex ovo CAM.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (NIH grant HL95678)en_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.mvr.2014.06.009en_US
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.sourcePMCen_US
dc.titleStretch-induced intussuceptive and sprouting angiogenesis in the chick chorioallantoic membraneen_US
dc.typeArticleen_US
dc.identifier.citationBelle, Janeil, Alexandra Ysasi, Robert D. Bennett, Nenad Filipovic, Mohammad Imani Nejad, David L. Trumper, Maximilian Ackermann, et al. “Stretch-Induced Intussuceptive and Sprouting Angiogenesis in the Chick Chorioallantoic Membrane.” Microvascular Research 95 (September 2014): 60–67.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.mitauthorImani Nejad, Mohammaden_US
dc.contributor.mitauthorTrumper, David L.en_US
dc.relation.journalMicrovascular Researchen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsBelle, Janeil; Ysasi, Alexandra; Bennett, Robert D.; Filipovic, Nenad; Nejad, Mohammad Imani; Trumper, David L.; Ackermann, Maximilian; Wagner, Willi; Tsuda, Akira; Konerding, Moritz A.; Mentzer, Steven J.en_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-8981-1912
dc.identifier.orcidhttps://orcid.org/0000-0001-5358-5450
mit.licensePUBLISHER_CCen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record