dc.contributor.author | Pollock, F. J | |
dc.contributor.author | Krediet, Cory J | |
dc.contributor.author | Winn, Karina | |
dc.contributor.author | Wilson, Bryan | |
dc.contributor.author | Huete-Stauffer, Carla | |
dc.contributor.author | Willis, Bette L | |
dc.contributor.author | Bourne, David G | |
dc.contributor.author | Pollock, F. Joseph | |
dc.contributor.author | Krediet, Cory J. | |
dc.contributor.author | Willis, Bette L. | |
dc.contributor.author | Bourne, David G. | |
dc.contributor.author | Stocker, Roman | |
dc.contributor.author | Garren, Melissa | |
dc.date.accessioned | 2016-10-03T18:35:56Z | |
dc.date.available | 2016-10-03T18:35:56Z | |
dc.date.issued | 2015-02 | |
dc.date.submitted | 2014-08 | |
dc.identifier.issn | 0722-4028 | |
dc.identifier.issn | 1432-0975 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/104638 | |
dc.description.abstract | The bacterium Vibrio coralliilyticus has been implicated as the causative agent of coral tissue loss diseases (collectively known as white syndromes) at sites across the Indo-Pacific and represents an emerging model pathogen for understanding the mechanisms linking bacterial infection and coral disease. In this study, we used a mini-Tn7 transposon delivery system to chromosomally label a strain of V. coralliilyticus isolated from a white syndrome disease lesion with a green fluorescent protein gene (GFP). We then tested the utility of this modified strain as a research tool for studies of coral host–pathogen interactions. A suite of biochemical assays and experimental infection trials in a range of model organisms confirmed that insertion of the GFP gene did not interfere with the labeled strain’s virulence. Using epifluorescence video microscopy, the GFP-labeled strain could be reliably distinguished from non-labeled bacteria present in the coral holobiont, and the pathogen’s interactions with the coral host could be visualized in real time. This study demonstrates that chromosomal GFP labeling is a useful technique for visualization and tracking of coral pathogens and provides a novel tool to investigate the role of V. coralliilyticus in coral disease pathogenesis. | en_US |
dc.description.sponsorship | Human Frontier Science Program (Strasbourg, France) (No. RGY0089RS) | en_US |
dc.publisher | Springer Berlin Heidelberg | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1007/s00338-015-1273-3 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
dc.source | Springer Berlin Heidelberg | en_US |
dc.title | Visualization of coral host--pathogen interactions using a stable GFP-labeled Vibrio coralliilyticus strain | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Pollock, F. Joseph, Cory J. Krediet, Melissa Garren, Roman Stocker, Karina Winn, Bryan Wilson, Carla Huete-Stauffer, Bette L. Willis, and David G. Bourne. “Visualization of Coral Host–pathogen Interactions Using a Stable GFP-Labeled Vibrio Coralliilyticus Strain.” Coral Reefs 34, no. 2 (February 26, 2015): 655–662. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering | en_US |
dc.contributor.department | Parsons Laboratory for Environmental Science and Engineering (Massachusetts Institute of Technology) | en_US |
dc.contributor.mitauthor | Garren, Melissa S. | |
dc.contributor.mitauthor | Stocker, Roman | |
dc.relation.journal | Coral Reefs | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2016-08-18T15:24:55Z | |
dc.language.rfc3066 | en | |
dc.rights.holder | Springer-Verlag Berlin Heidelberg | |
dspace.embargo.terms | N | en |
dc.identifier.orcid | https://orcid.org/0000-0002-3199-0508 | |
mit.license | OPEN_ACCESS_POLICY | en_US |
mit.metadata.status | Complete | |