dc.contributor.author | Truttmann, Matthias | |
dc.contributor.author | Cruz, Victor Emmanuel | |
dc.contributor.author | Guo, Xuanzong | |
dc.contributor.author | Engert, Christoph | |
dc.contributor.author | Schwartz, Thomas | |
dc.contributor.author | Ploegh, Hidde | |
dc.date.accessioned | 2016-12-09T19:51:39Z | |
dc.date.available | 2016-12-09T19:51:39Z | |
dc.date.issued | 2016-05 | |
dc.date.submitted | 2015-09 | |
dc.identifier.issn | 1553-7404 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/105785 | |
dc.description.abstract | Protein AMPylation by Fic domain-containing proteins (Fic proteins) is an ancient and conserved post-translational modification of mostly unexplored significance. Here we characterize the Caenorhabditis elegans Fic protein FIC-1 in vitro and in vivo. FIC-1 is an AMPylase that localizes to the nuclear surface and modifies core histones H2 and H3 as well as heat shock protein 70 family members and translation elongation factors. The three-dimensional structure of FIC-1 is similar to that of its human ortholog, HYPE, with 38% sequence identity. We identify a link between FIC-1-mediated AMPylation and susceptibility to the pathogen Pseudomonas aeruginosa, establishing a connection between AMPylation and innate immunity in C. elegans. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (P41 GM103403) | en_US |
dc.description.sponsorship | Swiss National Science Foundation (Advanced Postdoc Mobility Fellowship) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (NIH Pioneer Award (DP01)) | en_US |
dc.language.iso | en_US | |
dc.publisher | Public Library of Science | |
dc.relation.isversionof | http://dx.doi.org/10.1371/journal.pgen.1006023 | en_US |
dc.rights | Creative Commons Attribution 4.0 International License | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | PLOS | en_US |
dc.title | The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Truttmann, Matthias C., Victor E. Cruz, Xuanzong Guo, Christoph Engert, Thomas U. Schwartz, and Hidde L. Ploegh. “The Caenorhabditis Elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones.” Edited by Man-Wah Tan. PLOS Genetics 12, no. 5 (May 3, 2016): e1006023. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Computational and Systems Biology Program | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Whitehead Institute for Biomedical Research | en_US |
dc.contributor.mitauthor | Truttmann, Matthias | |
dc.contributor.mitauthor | Cruz, Victor Emmanuel | |
dc.contributor.mitauthor | Guo, Xuanzong | |
dc.contributor.mitauthor | Engert, Christoph | |
dc.contributor.mitauthor | Schwartz, Thomas | |
dc.contributor.mitauthor | Ploegh, Hidde | |
dc.relation.journal | PLOS Genetics | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Truttmann, Matthias C.; Cruz, Victor E.; Guo, Xuanzong; Engert, Christoph; Schwartz, Thomas U.; Ploegh, Hidde L. | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-5338-0973 | |
dc.identifier.orcid | https://orcid.org/0000-0001-6819-8832 | |
dc.identifier.orcid | https://orcid.org/0000-0001-8012-1512 | |
dc.identifier.orcid | https://orcid.org/0000-0002-1090-6071 | |
mit.license | PUBLISHER_CC | en_US |
mit.metadata.status | Complete | |