dc.contributor.author | Vinogradov, Alexander Alexandrovich | |
dc.contributor.author | Evans, Ethan Daniel | |
dc.contributor.author | Pentelute, Bradley L. | |
dc.date.accessioned | 2017-05-23T13:58:16Z | |
dc.date.available | 2017-05-23T13:58:16Z | |
dc.date.issued | 2015-02 | |
dc.date.submitted | 2014-12 | |
dc.identifier.issn | 2041-6520 | |
dc.identifier.issn | 2041-6539 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/109283 | |
dc.description.abstract | In this study we synthesized and characterized mirror image barnase (B. amyloliquefaciens ribonuclease). D-Barnase was identical to L-barnase, when analyzed by liquid chromatography and mass-spectrometry. Proteolysis of the mirror image enzyme revealed that in contrast to its native counterpart, D-barnase was completely stable to digestive proteases. In enzymatic assays, D-barnase had the reciprocal chiral specificity and was fully active towards mirror image substrates. Interestingly, D-barnase also hydrolyzed the substrate of the native chirality, albeit 4000 times less efficiently. This effect was further confirmed by digesting a native 112-mer RNA with the enzyme. Additional studies revealed that barnase accommodates a range of substrates with various chiralities, but the prime requirement for guanosine remains. These studies point toward using mirror image enzymes as modern agents in biotechnology. | en_US |
dc.description.sponsorship | United States. Defense Advanced Research Projects Agency (023504-001) | en_US |
dc.language.iso | en_US | |
dc.publisher | Royal Society of Chemistry, The | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1039/c4sc03877k | en_US |
dc.rights | Creative Commons Attribution 3.0 Unported licence | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/ | en_US |
dc.source | Royal Society of Chemistry | en_US |
dc.title | Total synthesis and biochemical characterization of mirror image barnase | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Vinogradov, Alexander A., Ethan D. Evans, and Bradley L. Pentelute. “Total Synthesis and Biochemical Characterization of Mirror Image Barnase.” Chemical Science 6, no. 5 (February 2015): 2997–3002 © 2015 The Royal Society of Chemistry | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemistry | en_US |
dc.contributor.mitauthor | Vinogradov, Alexander Alexandrovich | |
dc.contributor.mitauthor | Evans, Ethan Daniel | |
dc.contributor.mitauthor | Pentelute, Bradley L. | |
dc.relation.journal | Chemical Science | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Vinogradov, Alexander A.; Evans, Ethan D.; Pentelute, Bradley L. | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-5508-0963 | |
dc.identifier.orcid | https://orcid.org/0000-0002-9383-2185 | |
mit.license | PUBLISHER_CC | en_US |