dc.contributor.author | Patel, Nehal | |
dc.contributor.author | Mino-Kenudson, Mari | |
dc.contributor.author | Thayer, Sarah P. | |
dc.contributor.author | Liss, Andrew S. | |
dc.contributor.author | Saung, May Tun | |
dc.contributor.author | Sharei, Armon Reza | |
dc.contributor.author | Adalsteinsson, Viktor A. | |
dc.contributor.author | Cho, Nahyun | |
dc.contributor.author | Ruiz, Camilo A. | |
dc.contributor.author | Kirkpatrick, Jesse D. | |
dc.contributor.author | Langer, Robert S | |
dc.contributor.author | Jensen, Klavs F | |
dc.contributor.author | Love, John C | |
dc.contributor.author | Kamath, Tushar V. | |
dc.date.accessioned | 2017-10-02T18:34:58Z | |
dc.date.available | 2017-10-02T18:34:58Z | |
dc.date.issued | 2016-11 | |
dc.date.submitted | 2016-07 | |
dc.identifier.issn | 1613-6810 | |
dc.identifier.issn | 1613-6829 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/111674 | |
dc.description.abstract | Identifying and separating a subpopulation of cells from a heterogeneous mixture are essential elements of biological research. Current approaches require detailed knowledge of unique cell surface properties of the target cell population. A method is described that exploits size differences of cells to facilitate selective intracellular delivery using a high throughput microfluidic device. Cells traversing a constriction within this device undergo a transient disruption of the cell membrane that allows for cytoplasmic delivery of cargo. Unique constriction widths allow for optimization of delivery to cells of different sizes. For example, a 4 μm wide constriction is effective for delivery of cargo to primary human T-cells that have an average diameter of 6.7 μm. In contrast, a 6 or 7 μm wide constriction is best for large pancreatic cancer cell lines BxPc3 (10.8 μm) and PANC-1 (12.3 μm). These small differences in cell diameter are sufficient to allow for selective delivery of cargo to pancreatic cancer cells within a heterogeneous mixture containing T-cells. The application of this approach is demonstrated by selectively delivering dextran-conjugated fluorophores to circulating tumor cells in patient blood allowing for their subsequent isolation and genomic characterization. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant R01GM101420-01A1) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant P01CA117969) | en_US |
dc.description.sponsorship | National Cancer Institute (U.S.) (Grant P30-CA14051) | en_US |
dc.language.iso | en_US | |
dc.publisher | Wiley Blackwell | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1002/smll.201601155 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
dc.source | Prof. Love via Erja Kajosalo | en_US |
dc.title | A Size-Selective Intracellular Delivery Platform | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Saung, May Tun et al. “A Size-Selective Intracellular Delivery Platform.” Small 12, 42 (September 2016): 5873–5881 © 2016 WILEY-VCH Verlag | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemical Engineering | en_US |
dc.contributor.department | Koch Institute for Integrative Cancer Research at MIT | en_US |
dc.contributor.approver | Love, Christopher J. | en_US |
dc.contributor.mitauthor | Saung, May Tun | |
dc.contributor.mitauthor | Sharei, Armon Reza | |
dc.contributor.mitauthor | Adalsteinsson, Viktor A. | |
dc.contributor.mitauthor | Cho, Nahyun | |
dc.contributor.mitauthor | Kamath, Tushar | |
dc.contributor.mitauthor | Ruiz, Camilo A. | |
dc.contributor.mitauthor | Kirkpatrick, Jesse D. | |
dc.contributor.mitauthor | Langer, Robert S | |
dc.contributor.mitauthor | Jensen, Klavs F | |
dc.contributor.mitauthor | Love, John C | |
dc.relation.journal | Small | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Saung, May Tun; Sharei, Armon; Adalsteinsson, Viktor A.; Cho, Nahyun; Kamath, Tushar; Ruiz, Camilo; Kirkpatrick, Jesse; Patel, Nehal; Mino-Kenudson, Mari; Thayer, Sarah P.; Langer, Robert; Jensen, Klavs F.; Liss, Andrew S.; Love, J. Christopher | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-9099-9281 | |
dc.identifier.orcid | https://orcid.org/0000-0003-4555-2485 | |
dc.identifier.orcid | https://orcid.org/0000-0002-1487-9568 | |
dc.identifier.orcid | https://orcid.org/0000-0003-4255-0492 | |
dc.identifier.orcid | https://orcid.org/0000-0001-7192-580X | |
dc.identifier.orcid | https://orcid.org/0000-0003-0921-3144 | |
mit.license | OPEN_ACCESS_POLICY | en_US |
mit.metadata.status | Complete | |