dc.contributor.author | Shen, Zuolian | |
dc.contributor.author | Kang, Jinsuk | |
dc.contributor.author | Shakya, Arvind | |
dc.contributor.author | Tabaka, Marcin | |
dc.contributor.author | Jarboe, Elke A | |
dc.contributor.author | Regev, Aviv | |
dc.contributor.author | Tantin, Dean | |
dc.date.accessioned | 2017-10-05T18:21:10Z | |
dc.date.available | 2017-10-05T18:21:10Z | |
dc.date.issued | 2017-05 | |
dc.date.submitted | 2016-08 | |
dc.identifier.issn | 2050-084X | |
dc.identifier.uri | http://hdl.handle.net/1721.1/111796 | |
dc.description.abstract | Embryonic stem cells co-express Oct4 and Oct1, a related protein with similar DNA-binding specificity. To study the role of Oct1 in ESC pluripotency and transcriptional control, we constructed germline and inducible-conditional Oct1-deficient ESC lines. ESCs lacking Oct1 show normal appearance, self-renewal and growth but manifest defects upon differentiation. They fail to form beating cardiomyocytes, generate neurons poorly, form small, poorly differentiated teratomas, and cannot generate chimeric mice. Upon RA-mediated differentiation, Oct1-deficient cells induce lineage-appropriate developmentally poised genes poorly while lineage-inappropriate genes, including extra-embryonic genes, are aberrantly expressed. In ESCs, Oct1 co-occupies a specific set of targets with Oct4, but does not occupy differentially expressed developmental targets. Instead, Oct1 occupies these targets as cells differentiate and Oct4 declines. These results identify a dynamic interplay between Oct1 and Oct4, in particular during the critical window immediately after loss of pluripotency when cells make the earliest developmental fate decisions. | en_US |
dc.publisher | eLife Sciences Publications, Ltd | en_US |
dc.relation.isversionof | http://dx.doi.org/10.7554/eLife.20937 | en_US |
dc.rights | Creative Commons Attribution 4.0 International | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | eLife | en_US |
dc.title | Enforcement of developmental lineage specificity by transcription factor Oct1 | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Shen, Zuolian et al. “Enforcement of Developmental Lineage Specificity by Transcription Factor Oct1.” eLife 6 (May 2017): e20937 © 2017 Shen et al. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.mitauthor | Regev, Aviv | |
dc.relation.journal | eLife | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2017-08-21T13:26:14Z | |
dspace.orderedauthors | Shen, Zuolian; Kang, Jinsuk; Shakya, Arvind; Tabaka, Marcin; Jarboe, Elke A; Regev, Aviv; Tantin, Dean | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-8567-2049 | |
mit.license | PUBLISHER_CC | en_US |
mit.metadata.status | Complete | |