dc.contributor.author | Kind, Jop | |
dc.contributor.author | Pagie, Ludo | |
dc.contributor.author | de Vries, Sandra S. | |
dc.contributor.author | Nahidiazar, Leila | |
dc.contributor.author | Dey, Siddharth S. | |
dc.contributor.author | Bienko, Magda | |
dc.contributor.author | Zhan, Ye | |
dc.contributor.author | Lajoie, Bryan | |
dc.contributor.author | de Graaf, Carolyn A. | |
dc.contributor.author | Amendola, Mario | |
dc.contributor.author | Jalink, Kees | |
dc.contributor.author | Dekker, Job | |
dc.contributor.author | van Oudenaarden, Alexander | |
dc.contributor.author | van Steensel, Bas | |
dc.contributor.author | Fudenberg, Geoffrey | |
dc.contributor.author | Imakaev, Maksim Viktorovich | |
dc.contributor.author | Mirny, Leonid A | |
dc.date.accessioned | 2017-12-19T15:04:57Z | |
dc.date.available | 2017-12-19T15:04:57Z | |
dc.date.issued | 2015-09 | |
dc.date.submitted | 2015-06 | |
dc.identifier.issn | 0092-8674 | |
dc.identifier.issn | 1097-4172 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/112800 | |
dc.description.abstract | Mammalian interphase chromosomes interact with the nuclear lamina (NL) through hundreds of large lamina-associated domains (LADs). We report a method to map NL contacts genome-wide in single human cells. Analysis of nearly 400 maps reveals a core architecture consisting of gene-poor LADs that contact the NL with high cell-to-cell consistency, interspersed by LADs with more variable NL interactions. The variable contacts tend to be cell-type specific and are more sensitive to changes in genome ploidy than the consistent contacts. Single-cell maps indicate that NL contacts involve multivalent interactions over hundreds of kilobases. Moreover, we observe extensive intra-chromosomal coordination of NL contacts, even over tens of megabases. Such coordinated loci exhibit preferential interactions as detected by Hi-C. Finally, the consistency of NL contacts is inversely linked to gene activity in single cells and correlates positively with the heterochromatic histone modification H3K9me3. These results highlight fundamental principles of single-cell chromatin organization. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant R01 GM114190) | en_US |
dc.description.sponsorship | National Human Genome Research Institute (U.S.) (Grant R01 HG003143) | en_US |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1016/J.CELL.2015.08.040 | en_US |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs License | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
dc.source | PMC | en_US |
dc.title | Genome-wide Maps of Nuclear Lamina Interactions in Single Human Cells | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Kind, Jop et al. “Genome-Wide Maps of Nuclear Lamina Interactions in Single Human Cells.” Cell 163, 1 (September 2015): 134–147 © 2015 Elsevier Inc | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Physics | en_US |
dc.contributor.mitauthor | Fudenberg, Geoffrey | |
dc.contributor.mitauthor | Imakaev, Maksim Viktorovich | |
dc.contributor.mitauthor | Mirny, Leonid A | |
dc.relation.journal | Cell | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2017-12-18T21:03:09Z | |
dspace.orderedauthors | Kind, Jop; Pagie, Ludo; de Vries, Sandra S.; Nahidiazar, Leila; Dey, Siddharth S.; Bienko, Magda; Zhan, Ye; Lajoie, Bryan; de Graaf, Carolyn A.; Amendola, Mario; Fudenberg, Geoffrey; Imakaev, Maxim; Mirny, Leonid A.; Jalink, Kees; Dekker, Job; van Oudenaarden, Alexander; van Steensel, Bas | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-5905-6517 | |
dc.identifier.orcid | https://orcid.org/0000-0002-5320-2728 | |
dc.identifier.orcid | https://orcid.org/0000-0002-0785-5410 | |
mit.license | PUBLISHER_CC | en_US |