dc.contributor.author | Wu, Xiaoxian | |
dc.contributor.author | Shen, Liqiang | |
dc.contributor.author | Zhuang, Ningning | |
dc.contributor.author | Zhang, Yu | |
dc.contributor.author | Haakonsen, Diane Laure | |
dc.contributor.author | Sanderlin, Allen G | |
dc.contributor.author | Liu, Yue | |
dc.contributor.author | Laub, Michael T | |
dc.date.accessioned | 2018-05-14T19:49:49Z | |
dc.date.available | 2018-05-14T19:49:49Z | |
dc.date.issued | 2018-03 | |
dc.date.submitted | 2018-02 | |
dc.identifier.issn | 0305-1048 | |
dc.identifier.issn | 1362-4962 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/115371 | |
dc.description.abstract | Canonical bacterial transcription activators bind to non-transcribed promoter elements to increase transcription of their target genes. Here we report crystal structures of binary complexes comprising domains of Caulobacter crescentus GcrA, a noncanonical bacterial transcription factor, that support a novel mechanism for transcription activation through the preferential binding of methylated cis-regulatory elements and the promotion of open complex formation through an interaction with region 2 of the principal σ factor, σ70. We present crystal structures of the C-terminal, σ factor-interacting domain (GcrA-SID) in complex with domain 2 of σ70 (σ702), and the N-terminal, DNA-binding domain (GcrA-DBD) in complex with methylated double-stranded DNA (dsDNA). The structures reveal interactions essential for transcription activation and DNA recognition by GcrA. These structures, along with mutational analyses, support a mechanism of transcription activation in which GcrA associates with RNA polymerase (RNAP) prior to promoter binding through GcrA-SID, arming RNAP with a flexible GcrA-DBD. The RNAP-GcrA complex then binds and activates target promoters harboring a methylated GcrA binding site either upstream or downstream of the transcription start site. | en_US |
dc.publisher | Oxford University Press | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1093/nar/gky161 | en_US |
dc.rights | Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | en_US |
dc.source | Nucleic Acids Research | en_US |
dc.title | Structural insights into the unique mechanism of transcription activation by Caulobacter crescentus GcrA | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Wu, Xiaoxian et al. “Structural Insights into the Unique Mechanism of Transcription Activation by Caulobacter Crescentus GcrA.” Nucleic Acids Research 46, 6 (March 2018): 3245–3256 © 2018 The Author(s) | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.mitauthor | Haakonsen, Diane Laure | |
dc.contributor.mitauthor | Sanderlin, Allen G | |
dc.contributor.mitauthor | Liu, Yue | |
dc.contributor.mitauthor | Laub, Michael T | |
dc.relation.journal | Nucleic Acids Research | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2018-05-04T17:07:26Z | |
dspace.orderedauthors | Wu, Xiaoxian; Haakonsen, Diane L; Sanderlin, Allen G; Liu, Yue J; Shen, Liqiang; Zhuang, Ningning; Laub, Michael T; Zhang, Yu | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-4668-1695 | |
dc.identifier.orcid | https://orcid.org/0000-0002-8288-7607 | |
mit.license | PUBLISHER_CC | en_US |