Adaptive Response Enzyme AlkB Preferentially Repairs 1-Methylguanine and 3-Methylthymine Adducts in Double-Stranded DNA
Author(s)
Chen, Fangyi; Tang, Qi; Bian, Ke; Humulock, Zachary T.; Yang, Xuedong; Li, Deyu; Jost, Marco; Drennan, Catherine L.; Essigmann, John M.; ... Show more Show less
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The AlkB protein is a repair enzyme that uses an α-ketoglutarate/Fe(II)-dependent mechanism to repair alkyl DNA adducts. AlkB has been reported to repair highly susceptible substrates, such as 1-methyladenine and 3-methylcytosine, more efficiently in ss-DNA than in ds-DNA. Here, we tested the repair of weaker AlkB substrates 1-methylguanine and 3-methylthymine and found that AlkB prefers to repair them in ds-DNA. We also discovered that AlkB and its human homologues, ABH2 and ABH3, are able to repair the aforementioned adducts when the adduct is present in a mismatched base pair. These observations demonstrate the strong adaptability of AlkB toward repairing various adducts in different environments. (Chemical Equation Presented).
Date issued
2016-02Department
Massachusetts Institute of Technology. Center for Environmental Health Sciences; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of ChemistryJournal
Chemical Research in Toxicology
Publisher
American Chemical Society (ACS)
Citation
Chen, Fangyi et al. “Adaptive Response Enzyme AlkB Preferentially Repairs 1-Methylguanine and 3-Methylthymine Adducts in Double-Stranded DNA.” Chemical Research in Toxicology 29, 4 (March 2016): 687–693 © 2016 American Chemical Society
Version: Author's final manuscript
ISSN
0893-228X
1520-5010