dc.contributor.author | Woods, Stephanie | |
dc.contributor.author | Ek, Courtney | |
dc.contributor.author | Shen, Zeli | |
dc.contributor.author | Feng, Yan | |
dc.contributor.author | Ge, Zhongming | |
dc.contributor.author | Muthupalani, Sureshkumar | |
dc.contributor.author | Whary, Mark T | |
dc.contributor.author | Fox, James G | |
dc.date.accessioned | 2018-09-06T15:51:51Z | |
dc.date.available | 2018-09-06T15:51:51Z | |
dc.date.issued | 2016-05 | |
dc.identifier.issn | 1083-4389 | |
dc.identifier.issn | 1523-5378 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/117647 | |
dc.description.abstract | Background: Aged hamsters naturally infected with novel Helicobacter spp. classified in the H. bilis cluster develop hepatobiliary lesions and typhlocolitis. Methods: To determine whether enterohepatic H. spp. contribute to disease, Helicobacter-free hamsters were experimentally infected with H. spp. after suppression of intestinal bacteria by tetracycline treatment of dams and pups. After antibiotic withdrawal, weanlings were gavaged with four H. bilis-like Helicobacter spp. isolated from hamsters or H. bilis ATCC 43879 isolated from human feces and compared to controls (n = 7 per group). Results: Helicobacter bilis 43879-dosed hamsters were necropsied at 33 weeks postinfection (WPI) due to the lack of detectable infection by fecal PCR; at necropsy, 5 of 7 were weakly PCR positive but lacked intestinal lesions. The remaining hamsters were maintained for ~95 WPI; chronic H. spp. infection in hamsters (6/7) was confirmed by PCR, bacterial culture, fluorescent in situ hybridization, and ELISA. Hamsters had mild-to-moderate typhlitis, and three of the male H. spp.-infected hamsters developed small intestinal lymphoma, in contrast to one control. Of the three lymphomas in H. spp.-infected hamsters, one was a focal ileal mucosa-associated lymphoid tissue (MALT) B-cell lymphoma, while the other two were multicentric small intestinal large B-cell lymphomas involving both the MALT and extra-MALT mucosal sites with lymphoepithelial lesions. The lymphoma in the control hamster was a diffuse small intestinal lymphoma with a mixed population of T and B cells. Conclusions: Results suggest persistent H. spp. infection may augment risk for gastrointestinal MALT origin lymphomas. This model is consistent with H. pylori/heilmannii-associated MALT lymphoma in humans and could be further utilized to investigate the mechanisms of intestinal lymphoma development. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant T32-OD10978-26) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant R01-OD011141) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant P30-ES002109) | en_US |
dc.publisher | Wiley Blackwell | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1111/HEL.12265 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
dc.source | PMC | en_US |
dc.title | Male Syrian Hamsters Experimentally Infected with Helicobacter spp. of the H. bilis Cluster Develop MALT-Associated Gastrointestinal Lymphomas | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Woods, Stephanie E. et al. “Male Syrian Hamsters Experimentally Infected withHelicobacterspp. of theH. bilisCluster Develop MALT-Associated Gastrointestinal Lymphomas.” Helicobacter 21, 3 (September 2015): 201–217 © 2016 John Wiley & Sons Ltd | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Division of Comparative Medicine | en_US |
dc.contributor.mitauthor | Woods, Stephanie | |
dc.contributor.mitauthor | Ek, Courtney | |
dc.contributor.mitauthor | Shen, Zeli | |
dc.contributor.mitauthor | Feng, Yan | |
dc.contributor.mitauthor | Ge, Zhongming | |
dc.contributor.mitauthor | Muthupalani, Sureshkumar | |
dc.contributor.mitauthor | Whary, Mark T | |
dc.contributor.mitauthor | Fox, James G | |
dc.relation.journal | Helicobacter | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2018-08-29T16:24:45Z | |
dspace.orderedauthors | Woods, Stephanie E.; Ek, Courtney; Shen, Zeli; Feng, Yan; Ge, Zhongming; Muthupalani, Sureshkumar; Whary, Mark T.; Fox, James G. | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-9307-6116 | |
mit.license | OPEN_ACCESS_POLICY | en_US |