Browsing Synthetic Biology by Title

Now showing items 21-40 of 139

    • BBF RFC 21: BglBricks Assembly Standard 

      Anderson, J Christopher; Dueber, John E; Leguia, Mariana; Wu, Gabriel C; Goler, Jonathan A; e.a. (2009-09-18)
      The BglBricks standard has been developed as an alternative to the original XbaI/SpeI standard primarily to provide a solution to the issue of generating translational-fusion parts. When joined together in a standard ...

    • BBF RFC 26: In-Fusion BioBrick Assembly 

      Sleight, Sean (2009-07-09)
      This BioBricks Foundation Request for Comments (BBF RFC) describes a method to assemble two BioBricks using the Clontech In-Fusion PCR Cloning Kit while maintaining BioBrick standard formats.

    • BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI 

      Peisajovich, Sergio G.; Horwitz, Andrew; Hoeller, Oliver; Rhau, Benjamin; Lim, Wendell (2009-09-16)
      This BioBricks Foundation Request for Comments (BBF RFC) describes an alternative assembly standard based on the Type IIS restriction enzyme AarI.

    • BBF RFC 30: Draft of an RDF-based framework for the exchange and integration of Synthetic Biology data 

      Grunberg, Raik (2009-04-24)
      This Request for Comments (RFC) suggests a framework for the description, exchange and interlinking of Synthetic Biology data. The framework would create an open process for the evolution and “rolling” standardization ...

    • BBF RFC 36: Fusion protein BioBrick assembly standard with optional linker extension 

      Jerala, Roman (2009-09-01)
      This RFC 36 describes an extension of the original BioBrick assembly standard (BBF RFC 10) and Freiburg assembly standard (BBF RFC 25). The Fusion Assembly strategy described here is fully compatible with RFC 25 (Freiburg) ...

    • BBF RFC 37: Fusion protein BioBrick assembly standard with optional linker extension 

      Bencina, Mojca; Jerala, Roman (2009-09-02)
      This RFC 37 describes an extension of the original BioBrick assembly standard (BBF RFC 10) and Freiburg assembly standard (BBF RFC 25). The Fusion Assembly strategy described here is fully compatible with RFC 25 (Freiburg) ...

    • BBF RFC 38: RFC 38: Building Blocks - Standard Large DNA/Genome Construction 

      DiCarlo, James; Boeke, Jef (2009-10-07)
      The physical assembly of standard parts is currently a non-standard process, which can either come from direct genome PCR with restriction enzyme sites incorporated into the PCR primers, or overlap assembly PCR. ...

    • BBF RFC 47: BioBytes Assembly Standard 

      Ellison, Michael; Ridgway, Doug; Fedor, Justin; Garside, Erin; Robinson, Kelly; e.a. (2009-10-29)
      The BioBytes assembly method provides a new high-throughput way of assembling plasmids as well as other large DNA constructs. Using a bead-linked assembly of genetic parts with long (12 bp) standardized sticky ends, ...

    • BBF RFC 56: “Part Flavors” For Peptide-Coding Parts 

      Anderson, J. Chris (2010-10-11)
      “Part Flavors” are a means of describing the grammatical properties of parts. Specifically, this is introduced as a means of describing parts that encode peptides (CDS parts), but there may be value to extrapolating the ...

    • BBF RFC 94: Type IIS Assembly for Bacterial Transcriptional Units: A Standardized Assembly Method for Building Bacterial Transcriptional Units Using the Type IIS Restriction Enzymes BsaI and BbsI 

      Haddock, Traci L.; Densmore, Douglas M.; Appleton, Evan; Carr, Swati; Iverson, Sonya; e.a. (2015-08-28)
      This RFC94 describes an assembly standard based on the Type IIS restriction enzymes BsaI and BbsI (also called BpiI). This assembly standard is based upon the Modular Cloning (MoClo) assembly strategy, which was introduced ...

    • BBF RFC97: Genetic Circuit Standard 1.0 

      Wang, Lingjue; Zhu, Xiaodan; Li, Hanrun; He, Huiyu; Ni, Zhuangdian; e.a. (2013-10-29)
      Current, there is well adopted documentation of biobricks. The Registry of Standard Biology Part set the standard documentation for biobrick. However, there is no standard way to document a genetic circuit, what information ...

    • The BioBrick Public Agreement v1 (draft) 

      Fischer, Mark; Crews, Lee; Lynch, Jennifer; Schultz, Jason; Grewal, David; e.a. (2009-10-18)
      We present a draft for public comment of a new legal framework supporting the future of biotechnology. The framework, named here as the BioBrick Public Agreement, is based upon the contribution of promises not to assert ...

    • The BioBrick Public Agreement v1a 

      Endy, Drew; Grewal, David; Schultz, Jason; Lynch, Jennifer; Crews, Lee; e.a. (2010-01-25)
      We present an updated, clean draft for additional public comment of a new legal framework supporting the future of biotechnology. The framework, named here as the BioBrick Public Agreement, is based upon the contribution ...

    • BioBricks++: Simplifying Assembly of Standard DNA Components 

      Che, Austin (2004-06-09)
      Construction of complex biological systems can require assembling many modules together. However, existing assembly schemes are lacking in generality, ease of use, or power to perform some desirable operations. Currently, ...

    • BioJADE: A Design and Simulation Tool for Synthetic Biological Systems 

      Goler, Jonathan A. (2004-05-28)
      The next generations of both biological engineering and computer engineering demand that control be exerted at the molecular level. Creating, characterizing and controlling synthetic biological systems may provide us ...

    • "BioSandwich", a homology-based assembly method using a library of standard parts 

      Abbas, Yassen; Crossman, Allan; Fletcher, Eugene; French, Chris; Gibson, Clare; e.a. (2011-10-17)
      This Request for Comments (RFC) describes a strategy for using homology-based assembly methods to assemble parts from a library in any order.

    • Bioscaffold-Linker 

      Mina, Petros; Savery, Nigel (2009-10-21)
      Protein fusions are currently not supported by most assembly standards. This assembly standard aims to provide the basis of a solution to a biobrick-friendly protein fusion mechanism that supports the current favorite ...

    • Building Protein Domain Based Composite Biobricks for Mammalian Expression Systems 

      Balint, Balint L; Keret, Ophir; Brazda, Peter; Demeny, Mate; 2010 Debrecen-Hungary, iGEM (2010-12-05)
      The purpose of this RFC is to describe a method that allows the design of protein domain based parts, starting with gene centered information and translate these informations into BBF RFC 25 compatible part. The method is ...

    • Cellular computation and communications using engineered genetic regulatory networks 

      Weiss, Ron, 1970- (Massachusetts Institute of Technology, 2001)
      In this thesis, I present an engineering discipline for obtaining complex, predictable, and reliable cell behaviors by embedding biochemical logic circuits and programmed intercellular communications into cells. To accomplish ...

    • Cellular Computing, ISAT Summer Study, August 1996 

      Knight, Thomas; Matsudaira, Paul (2016-01-27)
      Create and exploit a novel technology for information processing and manufacturing by controlling processes in living cells.