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Analysis of Targeted and Combinatorial Approaches to Phage T7 Genome Generation

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dc.contributor.author Mallet, Alex
dc.date.accessioned 2007-02-06T16:41:27Z
dc.date.available 2007-02-06T16:41:27Z
dc.date.issued 2007-02-06T16:41:27Z
dc.identifier.uri http://hdl.handle.net/1721.1/35880
dc.description.abstract I performed computational analyses of various approaches to generating re- engineered versions of the genome of bacteriophage T7. I analyzed a proposed design for a re-engineered genome by examining conservation of T7 genes across related phages, and looking for RNA secondary structure arising from the re-engineered genome that might contribute to unwanted regulation. In addition, I proposed two methods of generating libraries of T7 genomes, and implemented simulations showing that the proposed methods are theoretically feasible. I conclude with thoughts on how to further validate my proposed approaches to genome generation, and suggest a specific high-throughput method of characterizing rebuilt genomes. en
dc.language.iso en_US en
dc.subject bacteriophage t7 en
dc.subject refactoring en
dc.title Analysis of Targeted and Combinatorial Approaches to Phage T7 Genome Generation en


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