Zebrafish promoter microarrays identify actively transcribed embryonic genes

dc.contributor.author	Wardle, Fiona C
dc.contributor.author	Odom, Duncan
dc.contributor.author	Bell, George W.
dc.contributor.author	Wiellette, Elizabeth L
dc.contributor.author	Herbolsheimer, Elizabeth
dc.contributor.author	Smith, James C
dc.contributor.author	Yuan, Bingbing B.
dc.contributor.author	Sive, Hazel L.
dc.contributor.author	Danford, Timothy William
dc.contributor.author	Young, Richard A.
dc.date.accessioned	2010-10-05T20:08:23Z
dc.date.available	2010-10-05T20:08:23Z
dc.date.issued	2006-08
dc.date.submitted	2006-04
dc.identifier.issn	1474-760X
dc.identifier.issn	1465-6914
dc.identifier.issn	1465-6906
dc.identifier.uri	http://hdl.handle.net/1721.1/58880
dc.description.abstract	We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis.	en_US
dc.description.sponsorship	Wellcome Trust	en_US
dc.description.sponsorship	European Molecular Biology Organization	en_US
dc.description.sponsorship	National Institutes of Health (Grant HG002668) (Grant DK070813) (Grant 2RO1 HD33472) (Grant 5RO1 MH59942)	en_US
dc.publisher	BioMed Central Ltd	en_US
dc.relation.isversionof	http://dx.doi.org/10.1186/gb-2006-7-8-r71	en_US
dc.rights	Creative Commons Attribution	en_US
dc.rights.uri	http://creativecommons.org/licenses/by/2.0	en_US
dc.source	BioMed Central Ltd	en_US
dc.title	Zebrafish promoter microarrays identify actively transcribed embryonic genes	en_US
dc.type	Article	en_US
dc.identifier.citation	Genome Biology. 2006 Aug 04;7(8):R71	en_US
dc.contributor.department	Whitehead Institute for Biomedical Research	en_US
dc.contributor.department	Massachusetts Institute of Technology. Department of Biology	en_US
dc.contributor.department	Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory	en_US
dc.contributor.mitauthor	Yuan, Bingbing B.
dc.contributor.mitauthor	Sive, Hazel L.
dc.contributor.mitauthor	Danford, Timothy William
dc.contributor.mitauthor	Young, Richard A.
dc.contributor.mitauthor	Bell, George W.
dc.relation.journal	Genome Biology	en_US
dc.identifier.mitlicense	PUBLISHER_CC	en_US
dc.eprint.version	Final published version	en_US
dc.identifier.pmid	16889661
dc.type.uri	http://purl.org/eprint/type/JournalArticle	en_US
eprint.status	http://purl.org/eprint/status/PeerReviewed	en_US
dc.date.updated	2010-09-03T16:14:25Z
dc.language.rfc3066	en
dc.rights.holder	Wardle et al.; licensee BioMed Central Ltd.
dspace.orderedauthors	Wardle, Fiona C; Odom, Duncan T; Bell, George W; Yuan, Bingbing; Danford, Timothy W; Wiellette, Elizabeth L; Herbolsheimer, Elizabeth; Sive, Hazel L; Young, Richard A; Smith, James C	en