miMeasure – a standard for miRNA binding site characterization in mammalian cells
Citable URI:
http://hdl.handle.net/1721.1/60092
| Title: | miMeasure – a standard for miRNA binding site characterization in mammalian cells |
| Author: | Adlung, Lorenz; Sabah, Jude Al; Bayer, Philipp; Berrens, Rebecca; Cristiano, Elena; Flocke, Lea; Kleinsorg, Stefan; Kolodziejczyk, Aleksandra; Kraemer, Stephen; Torre, Alejandro Macias; Mathur, Aastha; Mayilo, Dmytro; Neumann, Stefan; Niopek, Dominik; Pisa, Rudolf; Schad, Jan-Ulrich; Schumacher, Laura-Nadine; Uhlig, Thomas; Wu, Xiaoting; Keienburg, Jens; Boerner, Kathleen; Grimm, Dirk; Eils, Roland |
| Issue Date: | 2010-12-05 |
| Abstract: | This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, BBa_K337049) enabling rapid experimental characterization of any miRNA-BS of choice. We recommend a new standard unit, RKDU (relative knock-down unit) to describe the knock-down efficiency of a miRNA-BS in a specific cell type. pSMB_miMeasure allows for an easy and fast measurement of RKDU while providing effective normalization against variance stemming from differences in transfection efficiency and from other sources. |
| URI: | http://hdl.handle.net/1721.1/60092 |
| Series/Report no.: | BBF RFC;73 |
| Keywords: | measurement standard |
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| BBFRFC73.pdf | 384.5Kb |
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