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The AAA+ ClpX machine unfolds a keystone subunit to remodel the Mu transpososome

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dc.contributor.author Baker, Tania
dc.contributor.author Sauer, Robert T.
dc.contributor.author Abdelhakim, Aliaa H.
dc.date.accessioned 2011-03-01T20:16:06Z
dc.date.available 2011-03-01T20:16:06Z
dc.date.issued 2009-09
dc.date.submitted 2009-08
dc.identifier.issn 0027-8424
dc.identifier.issn 1091-6490
dc.identifier.uri http://hdl.handle.net/1721.1/61360
dc.description.abstract A hyperstable complex of the tetrameric MuA transposase with recombined DNA must be remodeled to allow subsequent DNA replication. ClpX, a AAA+ enzyme, fulfills this function by unfolding one transpososome subunit. Which MuA subunit is extracted, and how complex destabilization relates to establishment of the correct directionality (left to right) of Mu replication, is not known. Here, using altered-specificity MuA proteins/DNA sites, we demonstrate that transpososome destabilization requires preferential ClpX unfolding of either the catalytic-left or catalytic-right subunits, which make extensive intersubunit contacts in the tetramer. In contrast, ClpX recognizes the other two subunits in the tetramer much less efficiently, and their extraction does not substantially destabilize the complex. Thus, ClpX targets the most stable structural components of the complex. Left-end biased Mu replication is not, however, determined by ClpX’s intrinsic subunit preference. The specific targeting of a stabilizing “keystone subunit” within a complex for unfolding is an attractive general mechanism for remodeling by AAA+ enzymes. en_US
dc.description.sponsorship National Institutes of Health (U.S.) (Grant GM-49224) (Grant AI-16892) en_US
dc.language.iso en_US
dc.publisher National Academy of Sciences (U.S.) en_US
dc.relation.isversionof http://dx.doi.org/10.1073/pnas.0910905106 en_US
dc.rights Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. en_US
dc.source PNAS en_US
dc.title The AAA+ ClpX machine unfolds a keystone subunit to remodel the Mu transpososome en_US
dc.type Article en_US
dc.identifier.citation Abdelhakim, Aliaa H., Robert T. Sauer, and Tania A. Baker. “The AAA+ ClpX machine unfolds a keystone subunit to remodel the Mu transpososome.” Proceedings of the National Academy of Sciences 107.6 (2010): 2437 -2442. © 2011 by the National Academy of Sciences en_US
dc.contributor.department Massachusetts Institute of Technology. Department of Biology en_US
dc.contributor.approver Baker, Tania
dc.contributor.mitauthor Baker, Tania
dc.contributor.mitauthor Sauer, Robert T.
dc.contributor.mitauthor Abdelhakim, Aliaa H.
dc.relation.journal Proceedings of the National Academy of Sciences of the United States of America. (PNAS) en_US
dc.identifier.mitlicense PUBLISHER_POLICY en_US
dc.eprint.version Final published version en_US
dc.type.uri http://purl.org/eprint/type/JournalArticle en_US
eprint.status http://purl.org/eprint/status/PeerReviewed en_US
dspace.orderedauthors Abdelhakim, A. H.; Sauer, R. T.; Baker, T. A. en


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