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H2A.Z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions

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dc.contributor.author Ku, Manching
dc.contributor.author Rheinbay, Esther
dc.contributor.author Endoh, Mitsuhiro
dc.contributor.author Koseki, Haruhiko
dc.contributor.author Koche, Richard Patrick
dc.contributor.author Jaffe, Jacob D.
dc.contributor.author Carr, Steven A.
dc.contributor.author Bernstein, Bradley E.
dc.date.accessioned 2012-10-04T19:58:50Z
dc.date.available 2012-10-04T19:58:50Z
dc.date.issued 2012-10
dc.date.submitted 2012-07
dc.identifier.issn 1465-6906
dc.identifier.issn 1474-7596
dc.identifier.uri http://hdl.handle.net/1721.1/73623
dc.description.abstract Abstract Background The histone variant H2A.Z has been implicated in nucleosome exchange, transcriptional activation and Polycomb repression. However, the relationships among these seemingly disparate functions remain obscure. Results We mapped H2A.Z genome-wide in mammalian ES cells and neural progenitors. H2A.Z is deposited promiscuously at promoters and enhancers, and correlates strongly with H3K4 methylation. Accordingly, H2A.Z is present at poised promoters with bivalent chromatin and at active promoters with H3K4 methylation, but is absent from stably repressed promoters that are specifically enriched for H3K27 trimethylation. We also characterized post-translational modification states of H2A.Z, including a novel species dually-modified by ubiquitination and acetylation that is enriched at bivalent chromatin. Conclusions Our findings associate H2A.Z with functionally distinct genomic elements, and suggest that post-translational modifications may reconcile its contrasting locations and roles. en_US
dc.publisher BioMed Central Ltd en_US
dc.relation.isversionof http://dx.doi.org/10.1186/gb-2012-13-10-r85 en_US
dc.rights Creative Commons Attribution en_US
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_US
dc.source BioMed Central Ltd en_US
dc.title H2A.Z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions en_US
dc.type Article en_US
dc.identifier.citation Genome Biology. 2012 Oct 03;13(10):R85 en_US
dc.contributor.department Harvard University--MIT Division of Health Sciences and Technology en_US
dc.contributor.mitauthor Koche, Richard Patrick
dc.relation.journal Genome Biology en_US
dc.identifier.mitlicense PUBLISHER_CC en_US
dc.eprint.version Final published version en_US
dc.type.uri http://purl.org/eprint/type/JournalArticle en_US
eprint.status http://purl.org/eprint/status/PeerReviewed en_US
dc.date.updated 2012-10-03T19:13:32Z
dc.language.rfc3066 en
dc.rights.holder Manching Ku et al.; licensee BioMed Central Ltd.
dspace.orderedauthors Ku, Manching; Jaffe, Jacob D; Koche, Richard P; Rheinbay, Esther; Endoh, Mitsuhiro; Koseki, Haruhiko; Carr, Steven A; Bernstein, Bradley E en


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