dc.contributor.author | Rottigni, A. | |
dc.contributor.author | Carminati, M. | |
dc.contributor.author | Ferrari, G. | |
dc.contributor.author | Vahey, M. D. | |
dc.contributor.author | Voldman, Joel | |
dc.contributor.author | Sampietro, M. | |
dc.date.accessioned | 2012-10-12T14:43:11Z | |
dc.date.available | 2012-10-12T14:43:11Z | |
dc.date.issued | 2011-05 | |
dc.date.submitted | 2011-04 | |
dc.identifier.issn | 0277-786X | |
dc.identifier.uri | http://hdl.handle.net/1721.1/73919 | |
dc.description.abstract | Lab-on-a-chip systems have been attracting a growing attention for the perspective of miniaturization and portability of bio-chemical assays. Here we present a the design and characterization of a miniaturized, USB-powered, self-contained, 2-channel instrument for impedance sensing, suitable for label-free tracking and real-time detection of cells flowing in microfluidic channels. This original circuit features a signal generator based on a direct digital synthesizer, a transimpedance amplifier, an integrated square-wave lock-in coupled to a Σ▵ ADC converter, and a digital processing platform. Real-time automatic peak detection on two channels is implemented in a FPGA. System functionality has been tested with an electronic resistance modulator to simulate 1% impedance variation produced by cells, reaching a time resolution of 50μs (enabling a count rate of 2000 events/s) with an applied voltage as low as 200mV. Biological experiments have been carried out counting yeast cells. Statistical analysis of events is in agreement with the expected amplitude and time distributions. 2-channel yeast counting has been performed with concomitant dielectrophoretic cell separation, showing that this novel and ultra compact sensing system, thanks to the selectivity of the lock-in detector, is compatible with other AC electrical fields applied to the device. | en_US |
dc.description.sponsorship | Fondazione Fratelli Agostino ed Enrico Rocca (Progetto Roberto Rocca Fellowship) | en_US |
dc.description.sponsorship | Singapore-MIT Alliance for Research and Technology Center | en_US |
dc.description.sponsorship | National Science Foundation (U.S.) (Grant DBI 0852654) | en_US |
dc.language.iso | en_US | |
dc.publisher | SPIE | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1117/12.886709 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | SPIE | en_US |
dc.title | Handheld 2-channel impedimetric cell counting system with embedded real-time processing | en_US |
dc.type | Article | en_US |
dc.identifier.citation | A. Rottigni ; M. Carminati ; G. Ferrari ; M. D. Vahey ; J. Voldman ; M. Sampietro; Handheld 2-channel impedimetric cell counting system with embedded real-time processing. Proc. SPIE 8068, Bioelectronics, Biomedical, and Bioinspired Systems V; and Nanotechnology V, 80680S (May 03, 2011). © (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE) | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Research Laboratory of Electronics | en_US |
dc.contributor.mitauthor | Vahey, M. D. | |
dc.contributor.mitauthor | Voldman, Joel | |
dc.relation.journal | Proceedings of SPIE--the International Society for Optical Engineering; v. 8068 | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/ConferencePaper | en_US |
dspace.orderedauthors | Rottigni, A.; Carminati, M.; Ferrari, G.; Vahey, M. D.; Voldman, J.; Sampietro, M. | en |
dc.identifier.orcid | https://orcid.org/0000-0001-8898-2296 | |
mit.license | PUBLISHER_POLICY | en_US |
mit.metadata.status | Complete | |