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Handheld 2-channel impedimetric cell counting system with embedded real-time processing

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dc.contributor.author Rottigni, A.
dc.contributor.author Carminati, M.
dc.contributor.author Ferrari, G.
dc.contributor.author Vahey, M. D.
dc.contributor.author Voldman, Joel
dc.contributor.author Sampietro, M.
dc.date.accessioned 2012-10-12T14:43:11Z
dc.date.available 2012-10-12T14:43:11Z
dc.date.issued 2011-05
dc.date.submitted 2011-04
dc.identifier.issn 0277-786X
dc.identifier.uri http://hdl.handle.net/1721.1/73919
dc.description.abstract Lab-on-a-chip systems have been attracting a growing attention for the perspective of miniaturization and portability of bio-chemical assays. Here we present a the design and characterization of a miniaturized, USB-powered, self-contained, 2-channel instrument for impedance sensing, suitable for label-free tracking and real-time detection of cells flowing in microfluidic channels. This original circuit features a signal generator based on a direct digital synthesizer, a transimpedance amplifier, an integrated square-wave lock-in coupled to a Σ▵ ADC converter, and a digital processing platform. Real-time automatic peak detection on two channels is implemented in a FPGA. System functionality has been tested with an electronic resistance modulator to simulate 1% impedance variation produced by cells, reaching a time resolution of 50μs (enabling a count rate of 2000 events/s) with an applied voltage as low as 200mV. Biological experiments have been carried out counting yeast cells. Statistical analysis of events is in agreement with the expected amplitude and time distributions. 2-channel yeast counting has been performed with concomitant dielectrophoretic cell separation, showing that this novel and ultra compact sensing system, thanks to the selectivity of the lock-in detector, is compatible with other AC electrical fields applied to the device. en_US
dc.description.sponsorship Fondazione Fratelli Agostino ed Enrico Rocca (Progetto Roberto Rocca Fellowship) en_US
dc.description.sponsorship Singapore-MIT Alliance for Research and Technology Center en_US
dc.description.sponsorship National Science Foundation (U.S.) (Grant DBI 0852654) en_US
dc.language.iso en_US
dc.publisher SPIE en_US
dc.relation.isversionof http://dx.doi.org/10.1117/12.886709 en_US
dc.rights Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. en_US
dc.source SPIE en_US
dc.title Handheld 2-channel impedimetric cell counting system with embedded real-time processing en_US
dc.type Article en_US
dc.identifier.citation A. Rottigni ; M. Carminati ; G. Ferrari ; M. D. Vahey ; J. Voldman ; M. Sampietro; Handheld 2-channel impedimetric cell counting system with embedded real-time processing. Proc. SPIE 8068, Bioelectronics, Biomedical, and Bioinspired Systems V; and Nanotechnology V, 80680S (May 03, 2011). © (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE) en_US
dc.contributor.department Massachusetts Institute of Technology. Research Laboratory of Electronics en_US
dc.contributor.department Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science en_US
dc.contributor.mitauthor Vahey, M. D.
dc.contributor.mitauthor Voldman, Joel
dc.relation.journal Proceedings of SPIE--the International Society for Optical Engineering; v. 8068 en_US
dc.identifier.mitlicense PUBLISHER_POLICY en_US
dc.eprint.version Final published version en_US
dc.type.uri http://purl.org/eprint/type/ConferencePaper en_US
dspace.orderedauthors Rottigni, A.; Carminati, M.; Ferrari, G.; Vahey, M. D.; Voldman, J.; Sampietro, M. en


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