dc.contributor.author | Ploegh, Hidde | |
dc.contributor.author | Costantino, Cristina Maria | |
dc.contributor.author | Hafler, David A. | |
dc.date.accessioned | 2012-10-25T20:12:30Z | |
dc.date.available | 2012-10-25T20:12:30Z | |
dc.date.issued | 2009-06 | |
dc.date.submitted | 2009-03 | |
dc.identifier.issn | 0022-1767 | |
dc.identifier.issn | 1550-6606 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/74262 | |
dc.description | July 15, 2010 | en_US |
dc.description.abstract | Although it has long been known that human CD4+ T cells can express functional class II MHC molecules, the role of lysosomal proteases in the T cell class II MHC processing and presentation pathway is unknown. Using CD4+ T cell clones that constitutively express class II MHC, we determined that cathepsin S is necessary for invariant chain proteolysis in T cells. CD4+HLA-DR+ T cells down-regulated cathepsin S expression and activity 18 h after activation, thereby ceasing nascent class II MHC product formation. This blockade resulted in the loss of the invariant chain fragment CLIP from the cell surface, suggesting that—like professional APC—CD4+ HLA-DR+ cells modulate self-Ag presentation as a consequence of activation. Furthermore, cathepsin S expression and activity, and concordantly cell surface CLIP expression, was reduced in HLA-DR+ CD4+ T cells as compared with B cells both in vitro and ex vivo. | en_US |
dc.language.iso | en_US | |
dc.publisher | American Association of Immunologists | en_US |
dc.relation.isversionof | http://dx.doi.org/10.4049/jimmunol.0900921 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike 3.0 | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/ | en_US |
dc.source | PMC | en_US |
dc.title | Cathepsin S regulates class II MHC processing in human CD4+ HLA-DR+ T cells | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Costantino, C. M., H. L. Ploegh, and D. A. Hafler. “Cathepsin S Regulates Class II MHC Processing in Human CD4+ HLA-DR+ T Cells.” The Journal of Immunology 183.2 (2009): 945–952. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Whitehead Institute for Biomedical Research | en_US |
dc.contributor.mitauthor | Ploegh, Hidde | |
dc.relation.journal | Journal of Immunology | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Costantino, C. M.; Ploegh, H. L.; Hafler, D. A. | en |
dc.identifier.orcid | https://orcid.org/0000-0002-1090-6071 | |
mit.license | OPEN_ACCESS_POLICY | en_US |
mit.metadata.status | Complete | |