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Cathepsin S regulates class II MHC processing in human CD4+ HLA-DR+ T cells

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dc.contributor.author Ploegh, Hidde
dc.contributor.author Costantino, Cristina Maria
dc.contributor.author Hafler, David A.
dc.date.accessioned 2012-10-25T20:12:30Z
dc.date.available 2012-10-25T20:12:30Z
dc.date.issued 2009-06
dc.date.submitted 2009-03
dc.identifier.issn 0022-1767
dc.identifier.issn 1550-6606
dc.identifier.uri http://hdl.handle.net/1721.1/74262
dc.description July 15, 2010 en_US
dc.description.abstract Although it has long been known that human CD4+ T cells can express functional class II MHC molecules, the role of lysosomal proteases in the T cell class II MHC processing and presentation pathway is unknown. Using CD4+ T cell clones that constitutively express class II MHC, we determined that cathepsin S is necessary for invariant chain proteolysis in T cells. CD4+HLA-DR+ T cells down-regulated cathepsin S expression and activity 18 h after activation, thereby ceasing nascent class II MHC product formation. This blockade resulted in the loss of the invariant chain fragment CLIP from the cell surface, suggesting that—like professional APC—CD4+ HLA-DR+ cells modulate self-Ag presentation as a consequence of activation. Furthermore, cathepsin S expression and activity, and concordantly cell surface CLIP expression, was reduced in HLA-DR+ CD4+ T cells as compared with B cells both in vitro and ex vivo. en_US
dc.language.iso en_US
dc.publisher American Association of Immunologists en_US
dc.relation.isversionof http://dx.doi.org/10.4049/jimmunol.0900921 en_US
dc.rights Creative Commons Attribution-Noncommercial-Share Alike 3.0 en_US
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/ en_US
dc.source PMC en_US
dc.title Cathepsin S regulates class II MHC processing in human CD4+ HLA-DR+ T cells en_US
dc.type Article en_US
dc.identifier.citation Costantino, C. M., H. L. Ploegh, and D. A. Hafler. “Cathepsin S Regulates Class II MHC Processing in Human CD4+ HLA-DR+ T Cells.” The Journal of Immunology 183.2 (2009): 945–952. en_US
dc.contributor.department Massachusetts Institute of Technology. Department of Biology en_US
dc.contributor.department Whitehead Institute for Biomedical Research en_US
dc.contributor.mitauthor Ploegh, Hidde
dc.relation.journal Journal of Immunology en_US
dc.identifier.mitlicense OPEN_ACCESS_POLICY en_US
dc.eprint.version Author's final manuscript en_US
dc.type.uri http://purl.org/eprint/type/JournalArticle en_US
eprint.status http://purl.org/eprint/status/PeerReviewed en_US
dspace.orderedauthors Costantino, C. M.; Ploegh, H. L.; Hafler, D. A. en


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