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The structure of the scaffold nucleoporin Nup120 reveals a new and unexpected domain architecture

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dc.contributor.author Leksa, Nina Carolina
dc.contributor.author Brohawn, Stephen G.
dc.contributor.author Schwartz, Thomas U.
dc.date.accessioned 2012-11-01T19:50:54Z
dc.date.available 2012-11-01T19:50:54Z
dc.date.issued 2009-07
dc.date.submitted 2009-06
dc.identifier.uri http://hdl.handle.net/1721.1/74556
dc.description.abstract Nucleocytoplasmic transport is mediated by nuclear pore complexes (NPCs), enormous protein assemblies residing in circular openings in the nuclear envelope. The NPC is modular, with transient and stable components. The stable core is essentially built from two multiprotein complexes, the Y-shaped heptameric Nup84 complex and the Nic96 complex, arranged around an eightfold axis. We present the crystal structure of Nup120[subscript 1-757], one of the two short arms of the Y-shaped Nup84 complex. The protein adopts a compact oval shape built around a novel bipartite α-helical domain intimately integrated with a β-propeller domain. The domain arrangement is substantially different from the Nup85•Seh1 complex, which forms the other short arm of the Y. With the data presented here, we establish that all three branches of the Y-shaped Nup84 complex are tightly connected by helical interactions and that the β-propellers likely form interaction site(s) to neighboring complexes. en_US
dc.description.sponsorship National Institutes of Health (U.S.) (Grant GM77537) en_US
dc.description.sponsorship Pew Charitable Trusts (Scholar Award) en_US
dc.language.iso en_US
dc.publisher Elsevier en_US
dc.relation.isversionof http://dx.doi.org/10.1016/j.str.2009.06.003 en_US
dc.rights Creative Commons Attribution-Noncommercial-Share Alike 3.0 en_US
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/ en_US
dc.source PMC en_US
dc.title The structure of the scaffold nucleoporin Nup120 reveals a new and unexpected domain architecture en_US
dc.type Article en_US
dc.identifier.citation Leksa, Nina C., Stephen G. Brohawn, and Thomas U. Schwartz. “The Structure of the Scaffold Nucleoporin Nup120 Reveals a New and Unexpected Domain Architecture.” Structure 17.8 (2009): 1082–1091. en_US
dc.contributor.department Massachusetts Institute of Technology. Dept. of Biology en_US
dc.contributor.mitauthor Leksa, Nina Carolina
dc.contributor.mitauthor Brohawn, Stephen G.
dc.contributor.mitauthor Schwartz, Thomas U.
dc.relation.journal Structure en_US
dc.identifier.mitlicense OPEN_ACCESS_POLICY en_US
dc.eprint.version Author's final manuscript en_US
dc.type.uri http://purl.org/eprint/type/JournalArticle en_US
eprint.status http://purl.org/eprint/status/PeerReviewed en_US
dspace.orderedauthors Leksa, Nina C.; Brohawn, Stephen G.; Schwartz, Thomas U. en


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