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Molecular architecture of the Nup84–Nup145C–Sec13 edge element in the nuclear pore complex lattice

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dc.contributor.author Brohawn, Stephen G.
dc.contributor.author Schwartz, Thomas
dc.date.accessioned 2012-11-01T20:20:39Z
dc.date.available 2012-11-01T20:20:39Z
dc.date.issued 2009-10
dc.date.submitted 2009-07
dc.identifier.issn 1545-9993
dc.identifier.issn 1545-9985
dc.identifier.uri http://hdl.handle.net/1721.1/74558
dc.description.abstract Nuclear pore complexes (NPCs) facilitate all nucleocytoplasmic transport. These massive protein assemblies are modular, with a stable structural scaffold supporting more dynamically attached components. The scaffold is made from multiple copies of the heptameric Y complex and the heteromeric Nic96 complex. We previously showed that members of these core subcomplexes specifically share an ACE1 fold with Sec31 of the COPII vesicle coat, and we proposed a lattice model for the NPC based on this commonality. Here we present the crystal structure of the heterotrimeric 134-kDa complex of Nup84–Nup145C–Sec13 of the Y complex. The heterotypic ACE1 interaction of Nup84 and Nup145C is analogous to the homotypic ACE1 interaction of Sec31 that forms COPII lattice edge elements and is inconsistent with the alternative 'fence-like' NPC model. We construct a molecular model of the Y complex and compare the architectural principles of COPII and NPC lattices. en_US
dc.description.sponsorship National Institutes of Health (U.S.) (Grant GM77537) en_US
dc.description.sponsorship Pew Charitable Trusts (Scholar Award) en_US
dc.language.iso en_US
dc.publisher Nature Publishing Group en_US
dc.relation.isversionof http://dx.doi.org/10.1038/nsmb.1713 en_US
dc.rights Creative Commons Attribution-Noncommercial-Share Alike 3.0 en_US
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/ en_US
dc.source PMC en_US
dc.title Molecular architecture of the Nup84–Nup145C–Sec13 edge element in the nuclear pore complex lattice en_US
dc.type Article en_US
dc.identifier.citation Brohawn, Stephen G, and Thomas U Schwartz. “Molecular Architecture of the Nup84–Nup145C–Sec13 Edge Element in the Nuclear Pore Complex Lattice.” Nature Structural & Molecular Biology 16.11 (2009): 1173–1177. en_US
dc.contributor.department Massachusetts Institute of Technology. Department of Biology en_US
dc.contributor.mitauthor Brohawn, Stephen G.
dc.contributor.mitauthor Schwartz, Thomas
dc.relation.journal Nature Structural & Molecular Biology en_US
dc.identifier.mitlicense OPEN_ACCESS_POLICY en_US
dc.eprint.version Author's final manuscript en_US
dc.type.uri http://purl.org/eprint/type/JournalArticle en_US
eprint.status http://purl.org/eprint/status/PeerReviewed en_US
dspace.orderedauthors Brohawn, Stephen G; Schwartz, Thomas U en


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