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The primosomal protein DnaD inhibits cooperative DNA binding by the replication initiator DnaA in Bacillus subtilis

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dc.contributor.author Bonilla, Carla Y.
dc.contributor.author Grossman, Alan D.
dc.date.accessioned 2012-11-13T16:35:22Z
dc.date.available 2012-11-13T16:35:22Z
dc.date.issued 2012-07
dc.date.submitted 2012-05
dc.identifier.issn 0021-9193
dc.identifier.issn 1098-5530
dc.identifier.uri http://hdl.handle.net/1721.1/74629
dc.description.abstract DnaA is an AAA+ ATPase and the conserved replication initiator in bacteria. Bacteria control the timing of replication initiation by regulating the activity of DnaA. DnaA binds to multiple sites in the origin of replication (oriC) and is required for recruitment of proteins needed to load the replicative helicase. DnaA also binds to other chromosomal regions and functions as a transcription factor at some of these sites. Bacillus subtilis DnaD is needed during replication initiation for assembly of the replicative helicase at oriC and during replication restart at stalled replication forks. DnaD associates with DnaA at oriC and at other chromosomal regions bound by DnaA. Using purified proteins, we found that DnaD inhibited the ability of DnaA to bind cooperatively to DNA and caused a decrease in the apparent dissociation constant. These effects of DnaD were independent of the ability of DnaA to bind or hydrolyze ATP. Other proteins known to regulate B. subtilis DnaA also affect DNA binding, whereas much of the regulation of Escherichia coli DnaA affects nucleotide hydrolysis or exchange. We found that the rate of nucleotide exchange for B. subtilis DnaA was high and not affected by DnaD. The rapid exchange is similar to that of Staphylococcus aureus DnaA and in contrast to the low exchange rate of Escherichia coli DnaA. We suggest that organisms in which DnaA has a high rate of nucleotide exchange predominantly regulate the DNA binding activity of DnaA and that those with low rates of exchange regulate hydrolysis and exchange. en_US
dc.description.sponsorship United States. Public Health Service (Grant GM41934) en_US
dc.language.iso en_US
dc.publisher American Society for Microbiology en_US
dc.relation.isversionof http://dx.doi.org/10.1128/jb.00958-12 en_US
dc.rights Creative Commons Attribution-Noncommercial-Share Alike 3.0 en_US
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/ en_US
dc.source Grossman via Courtney Crummett en_US
dc.title The primosomal protein DnaD inhibits cooperative DNA binding by the replication initiator DnaA in Bacillus subtilis en_US
dc.type Article en_US
dc.identifier.citation Bonilla, C. Y., and A. D. Grossman. “The Primosomal Protein DnaD Inhibits Cooperative DNA Binding by the Replication Initiator DnaA in Bacillus Subtilis.” Journal of Bacteriology 194.18 (2012): 5110–5117. en_US
dc.contributor.department Massachusetts Institute of Technology. Department of Biology en_US
dc.contributor.approver Grossman, Alan
dc.contributor.mitauthor Bonilla, Carla Y.
dc.contributor.mitauthor Grossman, Alan D.
dc.relation.journal Journal of Bacteriology en_US
dc.identifier.mitlicense OPEN_ACCESS_POLICY en_US
dc.eprint.version Author's final manuscript en_US
dc.type.uri http://purl.org/eprint/type/JournalArticle en_US
eprint.status http://purl.org/eprint/status/PeerReviewed en_US
dspace.orderedauthors Bonilla, C. Y.; Grossman, A. D. en


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