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The Bacillus subtilis conjugative transposon ICEBs1 mobilizes plasmids lacking dedicated mobilization functions

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Show simple item record Lee, Catherine Ann Thomas, Jacob Grossman, Alan D. 2012-11-13T17:21:19Z 2012-11-13T17:21:19Z 2012-04 2012-02
dc.description.abstract Integrative and conjugative elements (ICEs, also known as conjugative transposons) are mobile elements that are found integrated in a host genome and can excise and transfer to recipient cells via conjugation. ICEs and conjugative plasmids are found in many bacteria and are important agents of horizontal gene transfer and microbial evolution. Conjugative elements are capable of self-transfer and also capable of mobilizing other DNA elements that are not able to self-transfer. Plasmids that can be mobilized by conjugative elements are generally thought to contain an origin of transfer (oriT), from which mobilization initiates, and to encode a mobilization protein (Mob, a relaxase) that nicks a site in oriT and covalently attaches to the DNA to be transferred. Plasmids that do not have both an oriT and a cognate mob are thought to be nonmobilizable. We found that Bacillus subtilis carrying the integrative and conjugative element ICEBs1 can transfer three different plasmids to recipient bacteria at high frequencies. Strikingly, these plasmids do not have dedicated mobilization-oriT functions. Plasmid mobilization required conjugation proteins of ICEBs1, including the putative coupling protein. In contrast, plasmid mobilization did not require the ICEBs1 conjugative relaxase or cotransfer of ICEBs1, indicating that the putative coupling protein likely interacts with the plasmid replicative relaxase and directly targets the plasmid DNA to the ICEBs1 conjugation apparatus. These results blur the current categorization of mobilizable and nonmobilizable plasmids and indicate that conjugative elements play a role in horizontal gene transfer even more significant than previously recognized. en_US
dc.description.sponsorship National Institutes of Health (U.S.) (Grant GM50895) en_US
dc.language.iso en_US
dc.publisher American Society for Microbiology en_US
dc.relation.isversionof en_US
dc.rights Creative Commons Attribution-Noncommercial-Share Alike 3.0 en_US
dc.rights.uri en_US
dc.source Grossman via Courtney Crummett en_US
dc.title The Bacillus subtilis conjugative transposon ICEBs1 mobilizes plasmids lacking dedicated mobilization functions en_US
dc.type Article en_US
dc.identifier.citation Lee, Catherine A., Jacob Thomas, and Alan D. Grossman. “The Bacillus subtilis Conjugative Transposon ICEBs1 Mobilizes Plasmids Lacking Dedicated Mobilization Functions.” Journal of Bacteriology 194.12 (2012): 3165-3172. en_US
dc.contributor.department Massachusetts Institute of Technology. Department of Biology en_US
dc.contributor.approver Grossman, Alan
dc.contributor.mitauthor Lee, Catherine Ann
dc.contributor.mitauthor Thomas, Jacob
dc.contributor.mitauthor Grossman, Alan D.
dc.relation.journal Journal of Bacteriology en_US
dc.identifier.mitlicense OPEN_ACCESS_POLICY en_US
dc.eprint.version Author's final manuscript en_US
dc.type.uri en_US
eprint.status en_US

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