Bioinspired Multivalent DNA Network for Capture and Release of Cells
Author(s)
Zhao, Weian; Cui, Cheryl; Bose, Suman; Guo, Dagang; Shen, Chong; Wong, Wesley P.; Halvorsen, Ken; Farokhzad, Omid C.; Teo, Grace Sock Leng; Phillips, Joseph A.; Dorfman, David M.; Karnik, Rohit; Karp, Jeffrey Michael; ... Show more Show less
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Capture and isolation of flowing cells and particulates from body fluids has enormous implications in diagnosis, monitoring, and drug testing, yet monovalent adhesion molecules used for this purpose result in inefficient cell capture and difficulty in retrieving the captured cells. Inspired by marine creatures that present long tentacles containing multiple adhesive domains to effectively capture flowing food particulates, we developed a platform approach to capture and isolate cells using a 3D DNA network comprising repeating adhesive aptamer domains that extend over tens of micrometers into the solution. The DNA network was synthesized from a microfluidic surface by rolling circle amplification where critical parameters, including DNA graft density, length, and sequence, could readily be tailored. Using an aptamer that binds to protein tyrosine kinase-7 (PTK7) that is overexpressed on many human cancer cells, we demonstrate that the 3D DNA network significantly enhances the capture efficiency of lymphoblast CCRF-CEM cells over monovalent aptamers and antibodies, yet maintains a high purity of the captured cells. When incorporated in a herringbone microfluidic device, the 3D DNA network not only possessed significantly higher capture efficiency than monovalent aptamers and antibodies, but also outperformed previously reported cell-capture microfluidic devices at high flow rates. This work suggests that 3D DNA networks may have broad implications for detection and isolation of cells and other bioparticles.
Date issued
2012-11Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science; Harvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Mechanical EngineeringJournal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
National Academy of Sciences (U.S.)
Citation
Zhao, W., C. H. Cui, S. Bose, et al. Bioinspired Multivalent DNA Network for Capture and Release of Cells. Proceedings of the National Academy of Sciences 109(48): 19626–19631, 2012.
Version: Final published version
ISSN
0027-8424
1091-6490