The stoichiometry of the nucleoporin 62 subcomplex of the nuclear pore in solution
Author(s)
Ulrich, Alexander; Partridge, James R.; Schwartz, Thomas
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The nuclear pore complex (NPC) regulates transport between the nucleus and cytoplasm. Soluble cargo-protein complexes navigate through the pore by binding to phenylalanine-glycine (FG)-repeat proteins attached to the channel walls. The Nup62 complex contains the FG-repeat proteins Nup62, Nup54, and Nup58 and is located in the center of the NPC. The three proteins bind each other via conserved coiled-coil segments. To determine the stoichiometry of the Nup62 complex, we undertook an in vitro study using gel filtration and analytical ultracentrifugation. Our results reveal a 1:1:1 stoichiometry of the Nup62 complex, where Nup54 is central with direct binding to Nup62 and Nup58. At high protein concentration, the complex forms larger assemblies while maintaining the Nup62:Nup54:Nup58 ratio. For the homologous Nsp1 complex from Saccharomyces cerevisiae, we determine the same stoichiometry, indicating evolutionary conservation. Furthermore, we observe that eliminating one binding partner can result in the formation of complexes with noncanonical stoichiometry, presumably because unpaired coiled-coil elements tend to find a promiscuous binding partner. We suggest that these noncanonical stoichiometries observed in vitro are unlikely to be physiologically relevant.
Date issued
2014-02Department
Massachusetts Institute of Technology. Department of BiologyJournal
Molecular Biology of the Cell
Publisher
American Society for Cell Biology
Citation
Ulrich, A., J. R. Partridge, and T. U. Schwartz. “The Stoichiometry of the Nucleoporin 62 Subcomplex of the Nuclear Pore in Solution.” Molecular Biology of the Cell 25, no. 9 (May 1, 2014): 1484–1492.
Version: Final published version
ISSN
1059-1524
1939-4586