dc.contributor.author | Tworoger, Michael B. | |
dc.contributor.author | Vasquez, Claudia G | |
dc.contributor.author | Martin, Adam C | |
dc.date.accessioned | 2015-04-02T18:26:02Z | |
dc.date.available | 2015-04-02T18:26:02Z | |
dc.date.issued | 2014-08 | |
dc.date.submitted | 2014-02 | |
dc.identifier.issn | 0021-9525 | |
dc.identifier.issn | 1540-8140 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/96347 | |
dc.description.abstract | Apical constriction is a cell shape change that promotes epithelial bending. Activation of nonmuscle myosin II (Myo-II) by kinases such as Rho-associated kinase (Rok) is important to generate contractile force during apical constriction. Cycles of Myo-II assembly and disassembly, or pulses, are associated with apical constriction during Drosophila melanogaster gastrulation. It is not understood whether Myo-II phosphoregulation organizes contractile pulses or whether pulses are important for tissue morphogenesis. Here, we show that Myo-II pulses are associated with pulses of apical Rok. Mutants that mimic Myo-II light chain phosphorylation or depletion of myosin phosphatase inhibit Myo-II contractile pulses, disrupting both actomyosin coalescence into apical foci and cycles of Myo-II assembly/disassembly. Thus, coupling dynamic Myo-II phosphorylation to upstream signals organizes contractile Myo-II pulses in both space and time. Mutants that mimic Myo-II phosphorylation undergo continuous, rather than incremental, apical constriction. These mutants fail to maintain intercellular actomyosin network connections during tissue invagination, suggesting that Myo-II pulses are required for tissue integrity during morphogenesis. | en_US |
dc.description.sponsorship | National Institute of General Medical Sciences (U.S.) (Transgenic RNAi Project at Harvard Medical School, (R01-GM084947)) | en_US |
dc.language.iso | en_US | |
dc.publisher | Rockefeller University Press | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1083/jcb.201402004 | en_US |
dc.rights | Creative Commons Attribution | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/ | en_US |
dc.source | Rockefeller University Press | en_US |
dc.title | Dynamic myosin phosphorylation regulates contractile pulses and tissue integrity during epithelial morphogenesis | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Vasquez, C. G., M. Tworoger, and A. C. Martin. “Dynamic Myosin Phosphorylation Regulates Contractile Pulses and Tissue Integrity During Epithelial Morphogenesis.” The Journal of Cell Biology 206, no. 3 (August 4, 2014): 435–450. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.mitauthor | Vasquez, Claudia G. | en_US |
dc.contributor.mitauthor | Tworoger, Michael B. | en_US |
dc.contributor.mitauthor | Martin, Adam C. | en_US |
dc.relation.journal | Journal of Cell Biology | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Vasquez, Claudia G.; Tworoger, Mike; Martin, Adam C. | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-8987-7508 | |
dc.identifier.orcid | https://orcid.org/0000-0001-8060-2607 | |
mit.license | PUBLISHER_CC | en_US |
mit.metadata.status | Complete | |