Integration of Solid-State Nanopores in Microfluidic Networks via Transfer Printing of Suspended Membranes
Author(s)
Jain, Tarun; Guerrero, Ricardo Jose S.; Aguilar, Carlos A.; Karnik, Rohit
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Solid-state nanopores have emerged as versatile single-molecule sensors for applications including DNA sequencing, protein unfolding, micro-RNA detection, label-free detection of single nucleotide polymorphisms, and mapping of DNA-binding proteins involved in homologous recombination. While machining nanopores in dielectric membranes provides nanometer-scale precision, the rigid silicon support for the membrane contributes capacitive noise and limits integration with microfluidic networks for sample preprocessing. Herein, we demonstrate a technique to directly transfer solid-state nanopores machined in dielectric membranes from a silicon support into a microfluidic network. The resulting microfluidic-addressable nanopores can sense single DNA molecules at high bandwidths and with low noise, owing to significant reductions in membrane capacitance. This strategy will enable large-scale integration of solid-state nanopores with microfluidic upstream and downstream processing and permit new functions with nanopores such as complex manipulations for multidimensional analysis and parallel sensing in two and three-dimensional architectures.
Date issued
2013-01Department
Lincoln Laboratory; Massachusetts Institute of Technology. Department of Mechanical EngineeringJournal
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Citation
Jain, Tarun, Ricardo Jose S. Guerrero, Carlos A. Aguilar, and Rohit Karnik. “Integration of Solid-State Nanopores in Microfluidic Networks via Transfer Printing of Suspended Membranes.” Analytical Chemistry 85, no. 8 (April 16, 2013): 3871–3878.
Version: Author's final manuscript
ISSN
0003-2700
1520-6882