Engineering intracellular biomineralization and biosensing by a magnetic protein
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Author(s)
Matsumoto, Yuri
Chen, Ritchie
Anikeeva, Polina Olegovna
Jasanoff, Alan Pradip
Date Issued
November 2015
Journal
Nature Communications
Publisher
Nature Publishing Group
Citation
Matsumoto, Yuri, Ritchie Chen, Polina Anikeeva, and Alan Jasanoff. “Engineering Intracellular Biomineralization and Biosensing by a Magnetic Protein.” Nat Comms 6 (November 2, 2015): 8721. © 2015 Macmillan Publishers Limited
Version
Final published version
Abstract
Remote measurement and manipulation of biological systems can be achieved using magnetic techniques, but a missing link is the availability of highly magnetic handles on cellular or molecular function. Here we address this need by using high-throughput genetic screening in yeast to select variants of the iron storage ferritin (Ft) that display enhanced iron accumulation under physiological conditions. Expression of Ft mutants selected from a library of 10[superscript 7] variants induces threefold greater cellular iron loading than mammalian heavy chain Ft, over fivefold higher contrast in magnetic resonance imaging, and robust retention on magnetic separation columns. Mechanistic studies of mutant Ft proteins indicate that improved magnetism arises in part from increased iron oxide nucleation efficiency. Molecular-level iron loading in engineered Ft enables detection of individual particles inside cells and facilitates creation of Ft-based intracellular magnetic devices. We demonstrate construction of a magnetic sensor actuated by gene expression in yeast.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Massachusetts Institute of Technology. Department of Nuclear Science and Engineering
Massachusetts Institute of Technology. Research Laboratory of Electronics
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DOI of Published Version
https://doi.org/10.1038/ncomms9721
