Gene co-regulation by Fezf2 selects neurotransmitter identity and connectivity of corticospinal neurons
Author(s)Lodato, Simona; Zuccaro, Emanuela; Chen, Hsu-Hsin; Yuan, Wen; Meleski, Alyssa; Takahashi, Emi; Mahony, Shaun; Gifford, David K; Arlotta, Paola; Molyneaux, Bradley J.; Goff, Loyal A.; Rinn, John L.; Gifford, David K.; ... Show more Show less
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The neocortex contains an unparalleled diversity of neuronal subtypes, each defined by distinct traits that are developmentally acquired under the control of subtype-specific and pan-neuronal genes. The regulatory logic that orchestrates the expression of these unique combinations of genes is unknown for any class of cortical neuron. Here, we report that Fezf2 is a selector gene able to regulate the expression of gene sets that collectively define mouse corticospinal motor neurons (CSMN). We find that Fezf2 directly induces the glutamatergic identity of CSMN via activation of Vglut1 (Slc17a7) and inhibits a GABAergic fate by repressing transcription of Gad1. In addition, we identify the axon guidance receptor EphB1 as a target of Fezf2 necessary to execute the ipsilateral extension of the corticospinal tract. Our data indicate that co-regulated expression of neuron subtype–specific and pan-neuronal gene batteries by a single transcription factor is one component of the regulatory logic responsible for the establishment of CSMN identity.
DepartmentMassachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Nature Publishing Group
Lodato, Simona, Bradley J Molyneaux, Emanuela Zuccaro, Loyal A Goff, Hsu-Hsin Chen, Wen Yuan, Alyssa Meleski, et al. “Gene Co-Regulation by Fezf2 Selects Neurotransmitter Identity and Connectivity of Corticospinal Neurons.” Nat Neurosci 17, no. 8 (July 6, 2014): 1046–1054.
Author's final manuscript