dc.contributor.author | Birch, Christina M. | |
dc.contributor.author | Hou, Han Wei | |
dc.contributor.author | Han, Jongyoon | |
dc.contributor.author | Niles, Jacquin | |
dc.date.accessioned | 2015-12-15T18:06:35Z | |
dc.date.available | 2015-12-15T18:06:35Z | |
dc.date.issued | 2015-07 | |
dc.date.submitted | 2015-02 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/100270 | |
dc.description.abstract | Plasmodium falciparum malaria parasites invade and remodel human red blood cells (RBCs) by trafficking parasite-synthesized proteins to the RBC surface. While these proteins mediate interactions with host cells that contribute to disease pathogenesis, the infected RBC surface proteome remains poorly characterized. Here we use a novel strategy (I-SELEX) to discover high affinity aptamers that selectively recognize distinct epitopes uniquely present on parasite-infected RBCs. Based on inertial focusing in spiral microfluidic channels, I-SELEX enables stringent partitioning of cells (efficiency ≥ 10[superscript 6]) from unbound oligonucleotides at high volume throughput (~2 × 10[superscript 6] cells min[superscript −1]). Using an RBC model displaying a single, non-native antigen and live malaria parasite-infected RBCs as targets, we establish suitability of this strategy for de novo aptamer selections. We demonstrate recovery of a diverse set of aptamers that recognize distinct, surface-displayed epitopes on parasite-infected RBCs with nanomolar affinity, including an aptamer against the protein responsible for placental sequestration, var2CSA. These findings validate I-SELEX as a broadly applicable aptamer discovery platform that enables identification of new reagents for mapping the parasite-infected RBC surface proteome at higher molecular resolution to potentially contribute to malaria diagnostics, therapeutics and vaccine efforts. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Director's New Innovator Award 1DP2OD007124) | en_US |
dc.description.sponsorship | Singapore-MIT Alliance for Research and Technology. Infectious Disease Interdisciplinary Research Group | en_US |
dc.description.sponsorship | Wade Fund | en_US |
dc.description.sponsorship | MIT Startup Funds | en_US |
dc.description.sponsorship | Singapore-MIT Alliance for Research and Technology. BioSystems and Micromechanics Interdisciplinary Research Group | en_US |
dc.description.sponsorship | National Science Foundation (U.S.). Graduate Research Fellowship | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Chemistry/Biology Interface Training Program Grant 1-T32-GM081081) | en_US |
dc.language.iso | en_US | |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/srep11347 | en_US |
dc.rights | Creative Commons Attribution 4.0 International License | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | Nature | en_US |
dc.title | Identification of malaria parasite-infected red blood cell surface aptamers by inertial microfluidic SELEX (I-SELEX) | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Birch, Christina M., Han Wei Hou, Jongyoon Han, and Jacquin C. Niles. “Identification of Malaria Parasite-Infected Red Blood Cell Surface Aptamers by Inertial Microfluidic SELEX (I-SELEX).” Scientific Reports 5 (July 1, 2015): 11347. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science | en_US |
dc.contributor.mitauthor | Birch, Christina M. | en_US |
dc.contributor.mitauthor | Hou, Han Wei | en_US |
dc.contributor.mitauthor | Han, Jongyoon | en_US |
dc.contributor.mitauthor | Niles, Jacquin | en_US |
dc.relation.journal | Scientific Reports | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Birch, Christina M.; Hou, Han Wei; Han, Jongyoon; Niles, Jacquin C. | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-7215-1439 | |
dc.identifier.orcid | https://orcid.org/0000-0002-6250-8796 | |
mit.license | PUBLISHER_CC | en_US |
mit.metadata.status | Complete | |