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dc.contributor.authorBraden, Gillian C.
dc.contributor.authorArbona, Rodolfo Ricart
dc.contributor.authorLepherd, Michelle
dc.contributor.authorMonette, Sebastien
dc.contributor.authorToma, Aziz
dc.contributor.authorFox, James G.
dc.contributor.authorDewhirst, Floyd E.
dc.contributor.authorLipman, Neil S.
dc.date.accessioned2015-12-28T00:31:01Z
dc.date.available2015-12-28T00:31:01Z
dc.date.issued2015-06
dc.date.submitted2014-12
dc.identifier.issn1532-0820
dc.identifier.urihttp://hdl.handle.net/1721.1/100517
dc.description.abstractDuring 1 year, experimentally naïve C57BL/6NCrl weanlings born to timed-pregnant dams from a single vendor demonstrated markedly increased mortality associated with runting, abnormal gait, and decreased activity. Gram-positive, aerobic, α-hemolytic, coccoid bacteria were isolated from the meninges (n = 16), blood (n = 1), and kidneys (n = 1) of clinically affected weanlings (n = 15); from the uterus (n = 1), meninges (n = 1), and oral cavity (n = 2) of 3 dams; and from the meninges and oral cavity of a clinically affected 86-d-old mouse in the same colony. Multifocal, necrosuppurative meningoencephalitis and ventriculitis with intralesional gram-positive coccoid bacteria were present in all but 2 affected animals. The bacterium also was isolated from the oral cavity of an asymptomatic timed-pregnant dam (1 of 23) from the same vendor and from 8 mice at the vendor's facility. All isolates (n = 25) were identified by using 2 semiautomated rapid-identification systems, one of which consistently identified the causative bacterium as Aerococcus viridans 2 (n = 12) or 3 (n = 13), with probabilities of 55.7% to 98.3%. The bacterium did not grow in 6.5% NaCl at 10 °C, thus suggesting a Streptococcus species. Partial 16S rRNA sequencing of 4 isolates suggested S. hyointestinalis (probability, 93.4%) and S. gallinaceus (99.5%). Full 16S rRNA sequences for 3 isolates identified the bacterium as a novel Streptococcus species most closely related to S. acidominimus strain LGM (96.5%) and Streptococcus species strain Smarlab 3301444 (96.3%) and for which we propose the name S. azizii.en_US
dc.language.isoen_US
dc.publisherAmerican Association for Laboratory Animal Scienceen_US
dc.relation.isversionofhttp://www.ingentaconnect.com/content/aalas/cm/2015/00000065/00000003/art00004?token=00491146b7e2a46762c6b66217d703c70533a3e5f7a673f7b2f27375f2a72752d70f1a671en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourceProf. Fox via Howard Silveren_US
dc.titleA novel α-hemolytic Streptococcus species (Streptococcus azizii sp. nov.) associated with meningoencephalitis in naïve weanling C57BL/6 miceen_US
dc.typeArticleen_US
dc.identifier.citationBraden, Gillian C., Rodolfo Ricart Arbona, Michelle Lepherd, Sebastien Monette, Aziz Toma, James G. Fox, Floyd E. Dewhirst, and Neil S. Lipman. "A novel α-hemolytic Streptococcus species (Streptococcus azizii sp. nov.) associated with meningoencephalitis in naïve weanling C57BL/6 mice." Comparative Medicine, Volume 65, Number 3 (June 2015).en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.approverFox, James G.en_US
dc.contributor.mitauthorFox, James G.en_US
dc.relation.journalComparative Medicineen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsBraden, Gillian C; Arbona, Rodolfo Ricart; Lepherd, Michelle; Monette, Sebastien; Toma, Aziz; Fox, James G.; Dewhirst, Floyd E.; Lipman, Neil S.en_US
dc.identifier.orcidhttps://orcid.org/0000-0001-9307-6116
mit.licenseOPEN_ACCESS_POLICYen_US


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