dc.contributor.author | Byun, Sangwon | |
dc.contributor.author | Begum, Shahinoor | |
dc.contributor.author | Miyamoto, David T. | |
dc.contributor.author | Maheswaran, Shyamala | |
dc.contributor.author | Stott, Shannon L. | |
dc.contributor.author | Toner, Mehmet | |
dc.contributor.author | Hecht, Vivian Chaya | |
dc.contributor.author | Hynes, Richard O | |
dc.contributor.author | Manalis, Scott R | |
dc.contributor.author | Shaw, Josephine | |
dc.date.accessioned | 2016-01-18T22:53:41Z | |
dc.date.available | 2016-01-18T22:53:41Z | |
dc.date.issued | 2015-12 | |
dc.date.submitted | 2015-07 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/100904 | |
dc.description.abstract | The potential for circulating tumor cells (CTCs) to elucidate the process of cancer metastasis and inform clinical decision-making has made their isolation of great importance. However, CTCs are rare in the blood, and universal properties with which to identify them remain elusive. As technological advancements have made single-cell deformability measurements increasingly routine, the assessment of physical distinctions between tumor cells and blood cells may provide insight into the feasibility of deformability-based methods for identifying CTCs in patient blood. To this end, we present an initial study assessing deformability differences between tumor cells and blood cells, indicated by the length of time required for them to pass through a microfluidic constriction. Here, we demonstrate that deformability changes in tumor cells that have undergone phenotypic shifts are small compared to differences between tumor cell lines and blood cells. Additionally, in a syngeneic mouse tumor model, cells that are able to exit a tumor and enter circulation are not required to be more deformable than the cells that were first injected into the mouse. However, a limited study of metastatic prostate cancer patients provides evidence that some CTCs may be more mechanically similar to blood cells than to typical tumor cell lines. | en_US |
dc.description.sponsorship | Janssen Pharmaceutical Ltd. | en_US |
dc.description.sponsorship | National Cancer Institute (U.S.). Physical Sciences Oncology Center (U54CA143874) | en_US |
dc.description.sponsorship | MIT-Harvard Center of Cancer Nanotechnology Excellence (Grant 26697290-47281-A) | en_US |
dc.description.sponsorship | Stand Up To Cancer | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.). P41 Biotechnology Resource Center | en_US |
dc.description.sponsorship | National Cancer Institute (U.S.) (Koch Institute Support Grant P30-CA14051) | en_US |
dc.language.iso | en_US | |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/srep18542 | en_US |
dc.rights | Creative Commons Attribution | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | Nature Publishing Group | en_US |
dc.title | Deformability of Tumor Cells versus Blood Cells | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Shaw Bagnall, Josephine, Sangwon Byun, Shahinoor Begum, David T. Miyamoto, Vivian C. Hecht, Shyamala Maheswaran, Shannon L. Stott, Mehmet Toner, Richard O. Hynes, and Scott R. Manalis. “Deformability of Tumor Cells Versus Blood Cells.” Scientific Reports 5 (December 18, 2015): 18542. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Mechanical Engineering | en_US |
dc.contributor.department | Koch Institute for Integrative Cancer Research at MIT | en_US |
dc.contributor.mitauthor | Bagnall, Josephine | en_US |
dc.contributor.mitauthor | Byun, Sangwon | en_US |
dc.contributor.mitauthor | Begum, Shahinoor | en_US |
dc.contributor.mitauthor | Hecht, Vivian Chaya | en_US |
dc.contributor.mitauthor | Hynes, Richard O. | en_US |
dc.contributor.mitauthor | Manalis, Scott R. | en_US |
dc.relation.journal | Scientific Reports | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Shaw Bagnall, Josephine; Byun, Sangwon; Begum, Shahinoor; Miyamoto, David T.; Hecht, Vivian C.; Maheswaran, Shyamala; Stott, Shannon L.; Toner, Mehmet; Hynes, Richard O.; Manalis, Scott R. | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-5223-9433 | |
dc.identifier.orcid | https://orcid.org/0000-0003-3415-3614 | |
dc.identifier.orcid | https://orcid.org/0000-0003-4110-1388 | |
dc.identifier.orcid | https://orcid.org/0000-0001-7603-8396 | |
mit.license | OPEN_ACCESS_POLICY | en_US |