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dc.contributor.authorChowdary, Praveen D.
dc.contributor.authorChe, Daphne L.
dc.contributor.authorKaplan, Luke
dc.contributor.authorChen, Ou
dc.contributor.authorPu, Kanyi
dc.contributor.authorCui, Bianxiao
dc.contributor.authorBawendi, Moungi G.
dc.date.accessioned2016-01-20T00:28:27Z
dc.date.available2016-01-20T00:28:27Z
dc.date.issued2015-12
dc.date.submitted2015-10
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/1721.1/100934
dc.description.abstractDynein-dependent transport of organelles from the axon terminals to the cell bodies is essential to the survival and function of neurons. However, quantitative knowledge of dyneins on axonal organelles and their collective function during this long-distance transport is lacking because current technologies to do such measurements are not applicable to neurons. Here, we report a new method termed nanoparticle-assisted optical tethering of endosomes (NOTE) that made it possible to study the cooperative mechanics of dyneins on retrograde axonal endosomes in live neurons. In this method, the opposing force from an elastic tether causes the endosomes to gradually stall under load and detach with a recoil velocity proportional to the dynein forces. These recoil velocities reveal that the axonal endosomes, despite their small size, can recruit up to 7 dyneins that function as independent mechanical units stochastically sharing load, which is vital for robust retrograde axonal transport. This study shows that NOTE, which relies on controlled generation of reactive oxygen species, is a viable method to manipulate small cellular cargos that are beyond the reach of current technology.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (DP2-NS082125)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (Award 1055112)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (Award 1344302)en_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/srep18059en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceNature Publishing Groupen_US
dc.titleNanoparticle-assisted optical tethering of endosomes reveals the cooperative function of dyneins in retrograde axonal transporten_US
dc.typeArticleen_US
dc.identifier.citationChowdary, Praveen D., Daphne L. Che, Luke Kaplan, Ou Chen, Kanyi Pu, Moungi Bawendi, and Bianxiao Cui. “Nanoparticle-Assisted Optical Tethering of Endosomes Reveals the Cooperative Function of Dyneins in Retrograde Axonal Transport.” Scientific Reports 5 (December 10, 2015): 18059.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemistryen_US
dc.contributor.mitauthorChen, Ouen_US
dc.contributor.mitauthorBawendi, Moungi G.en_US
dc.relation.journalScientific Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsChowdary, Praveen D.; Che, Daphne L.; Kaplan, Luke; Chen, Ou; Pu, Kanyi; Bawendi, Moungi; Cui, Bianxiaoen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-2220-4365
mit.licenseOPEN_ACCESS_POLICYen_US


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