MIT Libraries logoDSpace@MIT

MIT
View Item 
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Cooperative nutrient accumulation sustains growth of mammalian cells

Author(s)
Son, Sungmin; Stevens, Mark M.; Chao, Hui Xiao; Weng, Yaochung; Wood, Kris; Vander Heiden, Matthew G.; Hosios, Aaron Marc; Schweitzer, Lawrence David; Thoreen, Carson C; Sabatini, David; Manalis, Scott R; ... Show more Show less
Thumbnail
DownloadSon-2015-Cooperative nutrient.pdf (965.3Kb)
OPEN_ACCESS_POLICY

Open Access Policy

Creative Commons Attribution-Noncommercial-Share Alike

Terms of use
Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/
Metadata
Show full item record
Abstract
The coordination of metabolic processes to allow increased nutrient uptake and utilization for macromolecular synthesis is central for cell growth. Although studies of bulk cell populations have revealed important metabolic and signaling requirements that impact cell growth on long time scales, whether the same regulation influences short-term cell growth remains an open question. Here we investigate cell growth by monitoring mass accumulation of mammalian cells while rapidly depleting particular nutrients. Within minutes following the depletion of glucose or glutamine, we observe a growth reduction that is larger than the mass accumulation rate of the nutrient. This indicates that if one particular nutrient is depleted, the cell rapidly adjusts the amount that other nutrients are accumulated, which is consistent with cooperative nutrient accumulation. Population measurements of nutrient sensing pathways involving mTOR, AKT, ERK, PKA, MST1, or AMPK, or pro-survival pathways involving autophagy suggest that they do not mediate this growth reduction. Furthermore, the protein synthesis rate does not change proportionally to the mass accumulation rate over these time scales, suggesting that intracellular metabolic pools buffer the growth response. Our findings demonstrate that cell growth can be regulated over much shorter time scales than previously appreciated.
Date issued
2015-12
URI
http://hdl.handle.net/1721.1/100935
Department
Massachusetts Institute of Technology. Computational and Systems Biology Program; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Mechanical Engineering; Whitehead Institute for Biomedical Research; Koch Institute for Integrative Cancer Research at MIT
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Son, Sungmin, Mark M. Stevens, Hui Xiao Chao, Carson Thoreen, Aaron M. Hosios, Lawrence D. Schweitzer, Yaochung Weng, et al. “Cooperative Nutrient Accumulation Sustains Growth of Mammalian Cells.” Scientific Reports 5 (December 1, 2015): 17401.
Version: Final published version
ISSN
2045-2322

Collections
  • MIT Open Access Articles

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

Login

Statistics

OA StatisticsStatistics by CountryStatistics by Department
MIT Libraries
PrivacyPermissionsAccessibilityContact us
MIT
Content created by the MIT Libraries, CC BY-NC unless otherwise noted. Notify us about copyright concerns.