Engineering Escherichia coli coculture systems for the production of biochemical products
Author(s)Zhang, Haoran; Li, Zhengjun; Stephanopoulos, Gregory; Pereira, Brian J.
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Engineering microbial consortia to express complex biosynthetic pathways efficiently for the production of valuable compounds is a promising approach for metabolic engineering and synthetic biology. Here, we report the design, optimization, and scale-up of an Escherichia coli-E. coli coculture that successfully overcomes fundamental microbial production limitations, such as high-level intermediate secretion and low-efficiency sugar mixture utilization. For the production of the important chemical cis,cis-muconic acid, we show that the coculture approach achieves a production yield of 0.35 g/g from a glucose/xylose mixture, which is significantly higher than reported in previous reports. By efficiently producing another compound, 4-hydroxybenzoic acid, we also demonstrate that the approach is generally applicable for biosynthesis of other important industrial products.
DepartmentMassachusetts Institute of Technology. Department of Chemical Engineering
Proceedings of the National Academy of Sciences
National Academy of Sciences (U.S.)
Zhang, Haoran, Brian Pereira, Zhengjun Li, and Gregory Stephanopoulos. “Engineering Escherichia Coli Coculture Systems for the Production of Biochemical Products.” Proc Natl Acad Sci USA 112, no. 27 (June 25, 2015): 8266–8271.
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