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dc.contributor.authorDahlman, James E.
dc.contributor.authorKhan, Omar F.
dc.contributor.authorJhunjhunwala, Siddharth
dc.contributor.authorShaw, Taylor E.
dc.contributor.authorXing, Yiping
dc.contributor.authorSahay, Gaurav
dc.contributor.authorBader, Andrew
dc.contributor.authorBogorad, Roman L.
dc.contributor.authorYin, Hao
dc.contributor.authorDong, Yizhou
dc.contributor.authorJiang, Shan
dc.contributor.authorSeedorf, Danielle
dc.contributor.authorDave, Apeksha
dc.contributor.authorSandhu, Kamaljeet Singh
dc.contributor.authorWebber, Matthew
dc.contributor.authorRuda, Vera M.
dc.contributor.authorLytton-Jean, Abigail K. R.
dc.contributor.authorLevins, Christopher G.
dc.contributor.authorLanger, Robert
dc.contributor.authorAnderson, Daniel Griffith
dc.date.accessioned2016-02-09T15:35:33Z
dc.date.available2016-02-09T15:35:33Z
dc.date.issued2014-05
dc.date.submitted2013-08
dc.identifier.issn1748-3387
dc.identifier.issn1748-3395
dc.identifier.urihttp://hdl.handle.net/1721.1/101130
dc.description.abstractDysfunctional endothelium contributes to more diseases than any other tissue in the body. Small interfering RNAs (siRNAs) can help in the study and treatment of endothelial cells in vivo by durably silencing multiple genes simultaneously, but efficient siRNA delivery has so far remained challenging. Here, we show that polymeric nanoparticles made of low-molecular-weight polyamines and lipids can deliver siRNA to endothelial cells with high efficiency, thereby facilitating the simultaneous silencing of multiple endothelial genes in vivo. Unlike lipid or lipid-like nanoparticles, this formulation does not significantly reduce gene expression in hepatocytes or immune cells even at the dosage necessary for endothelial gene silencing. These nanoparticles mediate the most durable non-liver silencing reported so far and facilitate the delivery of siRNAs that modify endothelial function in mouse models of vascular permeability, emphysema, primary tumour growth and metastasis.en_US
dc.description.sponsorshipAmerican Society for Engineering Education. National Defense Science and Engineering Graduate Fellowshipen_US
dc.description.sponsorshipNational Science Foundation (U.S.)en_US
dc.description.sponsorshipMassachusetts Institute of Technology. Presidential Fellowshipen_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/nnano.2014.84en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleIn vivo endothelial siRNA delivery using polymeric nanoparticles with low molecular weighten_US
dc.typeArticleen_US
dc.identifier.citationDahlman, James E., Carmen Barnes, Omar F. Khan, Aude Thiriot, Siddharth Jhunjunwala, Taylor E. Shaw, Yiping Xing, et al. “In Vivo Endothelial siRNA Delivery Using Polymeric Nanoparticles with Low Molecular Weight.” Nature Nanotechnology 9, no. 8 (May 11, 2014): 648–655.en_US
dc.contributor.departmentInstitute for Medical Engineering and Scienceen_US
dc.contributor.departmentDavid H. Koch Institute for Integrative Cancer Research at MITen_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.mitauthorDahlman, James E.en_US
dc.contributor.mitauthorKhan, Omar F.en_US
dc.contributor.mitauthorJhunjhunwala, Siddharthen_US
dc.contributor.mitauthorShaw, Taylor E.en_US
dc.contributor.mitauthorXing, Yipingen_US
dc.contributor.mitauthorSahay, Gauraven_US
dc.contributor.mitauthorBader, Andrewen_US
dc.contributor.mitauthorBogorad, Roman L.en_US
dc.contributor.mitauthorYin, Haoen_US
dc.contributor.mitauthorDong, Yizhouen_US
dc.contributor.mitauthorJiang, Shanen_US
dc.contributor.mitauthorSeedorf, Danielleen_US
dc.contributor.mitauthorDave, Apekshaen_US
dc.contributor.mitauthorSandhu, Kamaljeet Singhen_US
dc.contributor.mitauthorWebber, Matthewen_US
dc.contributor.mitauthorRuda, Vera M.en_US
dc.contributor.mitauthorLytton-Jean, Abigail K. R.en_US
dc.contributor.mitauthorLevins, Christopher G.en_US
dc.contributor.mitauthorLanger, Roberten_US
dc.contributor.mitauthorAnderson, Daniel Griffithen_US
dc.relation.journalNature Nanotechnologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsDahlman, James E.; Barnes, Carmen; Khan, Omar F.; Thiriot, Aude; Jhunjunwala, Siddharth; Shaw, Taylor E.; Xing, Yiping; Sager, Hendrik B.; Sahay, Gaurav; Speciner, Lauren; Bader, Andrew; Bogorad, Roman L.; Yin, Hao; Racie, Tim; Dong, Yizhou; Jiang, Shan; Seedorf, Danielle; Dave, Apeksha; Singh Sandhu, Kamaljeet; Webber, Matthew J.; Novobrantseva, Tatiana; Ruda, Vera M.; Lytton-Jean, Abigail K. R.; Levins, Christopher G.; Kalish, Brian; Mudge, Dayna K.; Perez, Mario; Abezgauz, Ludmila; Dutta, Partha; Smith, Lynelle; Charisse, Klaus; Kieran, Mark W.; Fitzgerald, Kevin; Nahrendorf, Matthias; Danino, Dganit; Tuder, Rubin M.; von Andrian, Ulrich H.; Akinc, Akin; Panigrahy, Dipak; Schroeder, Avi; Koteliansky, Victor; Langer, Robert; Anderson, Daniel G.en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-2100-1171
dc.identifier.orcidhttps://orcid.org/0000-0001-5786-0659
dc.identifier.orcidhttps://orcid.org/0000-0001-5629-4798
dc.identifier.orcidhttps://orcid.org/0000-0001-6898-3793
dc.identifier.orcidhttps://orcid.org/0000-0003-3811-2369
dc.identifier.orcidhttps://orcid.org/0000-0001-8223-035X
dc.identifier.orcidhttps://orcid.org/0000-0003-0624-3532
dc.identifier.orcidhttps://orcid.org/0000-0001-8046-2288
dc.identifier.orcidhttps://orcid.org/0000-0003-4255-0492
mit.licenseOPEN_ACCESS_POLICYen_US


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