Nanoparticle-formulated siRNA targeting integrins inhibits hepatocellular carcinoma progression in mice
Author(s)Bogorad, Roman L.; Yin, Hao; Zeigerer, Anja; Nonaka, Hidenori; Ruda, Vera M.; Zerial, Marino; Koteliansky, Victor; Anderson, Daniel Griffith; ... Show more Show less
MetadataShow full item record
Integrins play an important role during development, regulating cell differentiation, proliferation and survival. Here we show that knockdown of integrin subunits slows down the progression of hepatocellular carcinoma (HCC). Using nanoparticulate delivery of short interfering RNAs targeting β1 and αv integrin subunits, we downregulate all integrin receptors in hepatocytes. Short-term integrin knockdown (2 weeks) does not cause apparent structural or functional perturbations of normal liver tissue. Alterations in liver morphology accumulate on sustained integrin downregulation (7 weeks). The integrin knockdown leads to significant retardation of HCC progression, reducing proliferation and increasing tumour cell death. This tumour retardation is accompanied by reduced activation of the MET oncogene as well as expression of its mature form on the cell surface. Our data suggest that transformed proliferating cells from HCC are more sensitive to knockdown of integrins than normal quiescent hepatocytes, highlighting the potential of small interfering RNA-mediated inhibition of integrins as an anti-cancer therapeutic approach.
DepartmentInstitute for Medical Engineering and Science; David H. Koch Institute for Integrative Cancer Research at MIT; Harvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Chemical Engineering
Nature Publishing Group
Bogorad, Roman L., Hao Yin, Anja Zeigerer, Hidenori Nonaka, Vera M. Ruda, Marino Zerial, Daniel G. Anderson, and Victor Koteliansky. “Nanoparticle-Formulated siRNA Targeting Integrins Inhibits Hepatocellular Carcinoma Progression in Mice.” Nat Comms 5 (May 21, 2014).
Author's final manuscript